Affinity bioelectroanalysis in cellular-level biomarker driven modern precision cancer diagnosis

Abstract

The increasingly prominent role played by precision, equitable and sustainable diagnosis, particularly of prevalent and highly heterogeneous diseases, based on the determination and characterization of biomarkers of cellular origin in liquid biopsy, such as whole cells, their secretomes and the exosomes they release, is unquestionable. Electrochemical biosensing and biosensors, mainly those involving affinity reactions, have emerged in recent years as competitive analytical alternatives to address these challenging targets versus other existing strategies in terms of simplicity, cost and compatibility with multiplexed or multi-omics point-of-care determinations. This review article aims to put on the table in a concise, accurate and updated way the fundamentals, versatility and opportunities provided by state-ofthe-art electrochemical biosensing (2018e2022) for the determination of these cellular-derived biomarkers. A more critical and personal view of the challenges to be faced in the shorter term and where future research and advances are expected to go is also given.