Cytoprotective and Anti-Oxidative Stress Capacity of South American Medicinal Plant Brunfelsia grandiflora in Endothelial Cells

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dc.contributor.authorRodríguez, José Luis
dc.contributor.authorClavo, Zoyla Mirella
dc.contributor.authorRamos González, Mariella
dc.contributor.authorDe la Cruz, Max
dc.contributor.authorInostroza Ruiz, Luis
dc.contributor.authorBada Laura, Wendy
dc.contributor.authorPalomino Ruiz-Poveda, Olga María
dc.contributor.authorFernández Alfonso, María Soledad
dc.contributor.authorMateos Briz, María Raquel
dc.contributor.authorGoya, Luis
dc.date.accessioned2024-05-28T12:45:19Z
dc.date.available2024-05-28T12:45:19Z
dc.date.issued2023-11-20
dc.descriptionAuthor Contributions: JLR and LG designed the research study. JLR, LG, ZMC, MDLC and LIR performed the research. MRG, WBL, OP, MSFA, RM provided help and advice on cell culture. MRG analyzed the data. LG, JLR and WBL wrote the manuscript. All authors contributed to editorial changes in the manuscript. All authors read and approved the final manuscript. All authors have participated sufficiently in the work and agreed to be accountable for all aspects of the work.
dc.description.abstractBackground: Oxidative stress is a potential cause of cardiovascular pathologies, so the protection of endothelial cells and vascular tissues is essential to avoid such conditions, perhaps with the use of natural compounds rich in phenolic compounds with a proven high antioxidant capacity. The present study was designed to show the cytoprotective capacity of phenolic extracts from the plant Brunfelsia grandiflora, as well as describe its antioxidant defense mechanisms and the expression of some molecular markers involved in cellular protection. Methods: Human EA.hy926 cells were exposed to Brunfelsia grandiflora (B. grandiflora) extract (1, 10, 25, 50, 100, and 200 μg/mL) in co-treatment (22 h with 100 μM tert-Butyl hydroperoxide (t-BOOH) and B. grandiflora concentrations) and pre-treatment (18 h of B. grandiflora concentrations and then 200 μM t-BOOH for 4 h). Cell viability, reactive oxygen species (ROS) production, nitric oxide (NO) levels, caspase 3/7 activity, malondialdehyde (MDA) concentration, and reduction in reduced glutathione (GSH) levels, glutathione peroxidase (GPx), and glutathione reductase (GR) activity were measured, and real-time PCR molecular assays superoxide dismutase (SOD2), nuclear factor E2-related factor (NRF2), BCL-2-associated X protein (BAX), and B-cell lymphoma 2 (BCL2) were performed. Data were analyzed via one-way ANOVA followed by Tukey's post hoc test. Results: B. grandiflora bark extract, mainly at concentrations of 25, 50, 100, and 200 μg/mL, significantly (p < 0.05) reversed (pre-treatment and co-treatment) the deleterious effects of t-BOOH on EA.hy926 endothelial cells, which were significantly decreased cell viability, GSH activity, and SOD2 and NRF2 expression (p < 0.05); and significantly increased levels of ROS, NO, MDA, caspase-3/7 activity, GPx activity, GR activity, and the BAX/BCL2 ratio (p < 0.05). Conclusions: B. grandiflora extract was able to reduce the deleterious effects of t-BOOH on EA.hy926 endothelial cells, which may indicate its potential phytotherapeutic benefit against cytotoxic damage caused by chemical agents.
dc.description.departmentDepto. de Farmacología, Farmacognosia y Botánica
dc.description.departmentSección Deptal. de Farmacología y Toxicología (Veterinaria)
dc.description.facultyFac. de Farmacia
dc.description.fundingtypeAPC financiada por la UCM
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía, Industria y Competitividad (España)
dc.description.sponsorshipUniversidad Nacional de San Marcos
dc.description.statuspub
dc.identifier.citationJosé-Luis Rodríguez , Zoyla-Mirella Clavo , Mariella Ramos-Gonzalez , Max De La Cruz , Luis Inostroza-Ruiz , Wendy Bada-Laura , Olga Palomino , Maria S. Fernández-Alfonso , Raquel Mateos , Luis Goya. Cytoprotective and Anti-Oxidative Stress Capacity of South American Medicinal Plant Brunfelsia grandiflora in Endothelial Cells. Journal of Biological Regulators and Homeostatic Agents. 2023, 37(11): 6179-6192 https://doi.org/10.23812/j.biol.regul.homeost.agents.20233711.590
dc.identifier.doi10.23812/j.biol.regul.homeost.agents.20233711.590
dc.identifier.essn1724-6083
dc.identifier.issn0393-974X
dc.identifier.officialurlhttps://doi.org/10.23812/j.biol.regul.homeost.agents.20233711.590
dc.identifier.urihttps://hdl.handle.net/20.500.14352/104499
dc.issue.number11
dc.journal.titleJournal of Biological Regulators and Homeostatic Agents
dc.language.isoeng
dc.page.final6192
dc.page.initial6179
dc.publisherBiolife
dc.relation.projectIDinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2017-82565-C2-2-R/ES/CROSSTALK ENTRE LOS ASTROCITOS HIPOTALAMICOS Y EL TEJIDO ADIPOSO PERIVASCULAR EN EL METABOLISMO ENERGETICO Y LA FUNCION CARDIOVASCULAR: IMPACTO DE MODIFICACIONES EN LA DIETA/
dc.relation.projectIDPCONFIGI A23040181
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.cdu615.1
dc.subject.keywordMedicinal plants
dc.subject.keywordB. grandiflora
dc.subject.keywordVascular endothelium
dc.subject.keywordOxidative stress
dc.subject.ucmBotánica (Farmacia)
dc.subject.ucmFarmacología (Farmacia)
dc.subject.unesco3109.08 Farmacología
dc.subject.unesco2417.03 Botánica General
dc.titleCytoprotective and Anti-Oxidative Stress Capacity of South American Medicinal Plant Brunfelsia grandiflora in Endothelial Cells
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number37
dspace.entity.typePublication
relation.isAuthorOfPublication55f38dac-c329-406e-830a-ab48763795fb
relation.isAuthorOfPublication880f080c-4a40-467a-bec8-2dbddbbea997
relation.isAuthorOfPublication06326d81-a768-4689-b448-485e235d8797
relation.isAuthorOfPublication.latestForDiscovery55f38dac-c329-406e-830a-ab48763795fb
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