Mg2+-linked self-assembly of FtsZ in the presence of GTP or a GTP analogue involves the concerted formation of a narrow size distribution of oligomeric species
dc.contributor.author | Monterroso, Begoña | |
dc.contributor.author | Ahijado Guzmán, Rubén | |
dc.contributor.author | Reija, Belén | |
dc.contributor.author | Alfonso, Carlos | |
dc.contributor.author | Zorrilla, Silvia | |
dc.contributor.author | Minton, Allen | |
dc.contributor.author | Rivas, Germán | |
dc.date.accessioned | 2024-01-09T16:57:11Z | |
dc.date.available | 2024-01-09T16:57:11Z | |
dc.date.issued | 2012 | |
dc.description.abstract | The assembly of the bacterial cell division FtsZ protein in the presence of constantly replenished GTP was studied as a function of Mg2+ concentration (at neutral pH and 0.5 M potassium) under steady-state conditions by sedimentation velocity, concentration-gradient light scattering, fluorescence correlation spectroscopy, and dynamic light scattering. Sedimentation velocity measurements confirmed previous results indicating cooperative appearance of a narrow size distribution of finite oligomers with increasing protein concentration. The concentration dependence of light scattering and diffusion coefficients independently verified the cooperative appearance of a narrow distribution of high molecular weight oligomers, and in addition provided a measurement of the average size of these species, which corresponds to 100 ± 20 FtsZ protomers at millimolar Mg2+ concentration. | |
dc.description.department | Depto. de Química Física | |
dc.description.faculty | Fac. de Ciencias Químicas | |
dc.description.refereed | TRUE | |
dc.description.status | pub | |
dc.identifier.citation | Begoña Monterroso, Rubén Ahijado-Guzmán, Belén Reija, Carlos Alfonso, Silvia Zorrilla, Allen P. Minton, and Germán Rivas Biochemistry 2012 51 (22), 4541-4550 DOI: 10.1021/bi300401b | |
dc.identifier.doi | 10.1021/bi300401b | |
dc.identifier.issn | 0006-2960 | |
dc.identifier.officialurl | https://doi.org/10.1021/bi300401b | |
dc.identifier.uri | https://hdl.handle.net/20.500.14352/92115 | |
dc.issue.number | 51 | |
dc.journal.title | Biochemistry | |
dc.language.iso | eng | |
dc.page.final | 4550 | |
dc.page.initial | 4541 | |
dc.publisher | ACS Publications | |
dc.rights.accessRights | restricted access | |
dc.subject.cdu | 544 | |
dc.subject.ucm | Ciencias Biomédicas | |
dc.subject.ucm | Química física (Química) | |
dc.subject.unesco | 2307 Química Física | |
dc.title | Mg2+-linked self-assembly of FtsZ in the presence of GTP or a GTP analogue involves the concerted formation of a narrow size distribution of oligomeric species | |
dc.type | journal article | |
dc.type.hasVersion | VoR | |
dspace.entity.type | Publication | |
relation.isAuthorOfPublication | 4af13c82-c400-4bf3-9103-829be26b4d63 | |
relation.isAuthorOfPublication.latestForDiscovery | 4af13c82-c400-4bf3-9103-829be26b4d63 |
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