Relevance of Biofilm Models in Periodontal Research: From Static to Dynamic Systems

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dc.contributor.authorSánchez Beltrán, María Del Carmen
dc.contributor.authorAlonso Español, Andrea
dc.contributor.authorRibeiro Vidal, Honorato José
dc.contributor.authorAlonso Álvarez, Bettina María
dc.contributor.authorHerrera González, David
dc.contributor.authorSanz Alonso, Mariano
dc.date.accessioned2023-06-17T08:31:37Z
dc.date.available2023-06-17T08:31:37Z
dc.date.issued2021
dc.descriptionThis research was part of the activities of Dentaid Extraordinary Chair in Periodontal Research (Cátedra Extraordinaria Dentaid en Investigación Periodontal, University Complutense of Madrid, Spain).en
dc.description.abstractMicrobial biofilm modeling has improved in sophistication and scope, although only a limited number of standardized protocols are available. This review presents an example of a biofilm model, along with its evolution and application in studying periodontal and peri-implant diseases. In 2011, the ETEP (Etiology and Therapy of Periodontal and Peri-Implant Diseases) research group at the University Complutense of Madrid developed an in vitro biofilm static model using representative bacteria from the subgingival microbiota, demonstrating a pattern of bacterial colonization and maturation similar to in vivo subgingival biofilms. When the model and its methodology were standardized, the ETEP research group employed the validated in vitro biofilm model for testing in different applications. The evolution of this model is described in this manuscript, from the mere observation of biofilm growth and maturation on static models on hydroxyapatite or titanium discs, to the evaluation of the impact of dental implant surface composition and micro-structure using the dynamic biofilm model. This evolution was based on reproducing the ideal microenvironmental conditions for bacterial growth within a bioreactor and reaching the target surfaces using the fluid dynamics mimicking the salivary flow. The development of this relevant biofilm model has become a powerful tool to study the essential processes that regulate the formation and maturation of these important microbial communities, as well as their behavior when exposed to different antimicrobial compounds.en
dc.description.departmentDepto. de Medicina
dc.description.facultyFac. de Medicina
dc.description.refereedTRUE
dc.description.sponsorshipUniversidad Complutense de Madrid
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/77357
dc.identifier.citationSánchez Beltrán, M. C., Alonso Español, A. Ribeiro Vidal, H. J. et al. «Relevance of Biofilm Models in Periodontal Research: From Static to Dynamic Systems». Microorganisms, vol. 9, n.o 2, febrero de 2021, p. 428. DOI.org (Crossref), https://doi.org/10.3390/microorganisms9020428.
dc.identifier.doi10.3390/microorganisms9020428
dc.identifier.issn2076-2607
dc.identifier.officialurlhttps://doi.org/10.3390/microorganisms9020428
dc.identifier.relatedurlhttps://www.mdpi.com/2076-2607/9/2/428
dc.identifier.urihttps://hdl.handle.net/20.500.14352/7366
dc.issue.number2
dc.journal.titleMicroorganisms
dc.language.isoeng
dc.page.initial428
dc.publisherMDPI
dc.relation.projectIDCátedra Extraordinaria Dentaid en Investigación Periodontal
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/es/
dc.subject.cdu616.314
dc.subject.keywordOral biofilms
dc.subject.keywordConfocal laser microscopy
dc.subject.keywordScanning electron microscopy
dc.subject.keywordBiofilm models
dc.subject.keywordDynamic systems
dc.subject.keywordRobbins device
dc.subject.keywordPeriodontal diseases
dc.subject.keywordPeri-implant diseases
dc.subject.keywordDental implants
dc.subject.ucmOdontología (Odontología)
dc.subject.unesco3213.13 Ortodoncia-Estomatología
dc.titleRelevance of Biofilm Models in Periodontal Research: From Static to Dynamic Systemsen
dc.typejournal article
dc.volume.number9
dspace.entity.typePublication
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