Assessment of the sensitivity and specificity of serological (IFAT) and molecular (direct PCR) techniques for diagnosis of leishmaniasis in lagomorphs using a Bayesian approach

dc.contributor.authorCruz Conty, María Luisa de la
dc.contributor.authorPérez, Andres
dc.contributor.authorDomínguez, Mercedes
dc.contributor.authorMoreno, Inmaculada
dc.contributor.authorGarcía Benzaquén, Nerea
dc.contributor.authorMartínez Alares, Irene
dc.contributor.authorNavarro Gómez, Alejandro
dc.contributor.authorÁlvarez, Julio
dc.contributor.authorDomínguez Rodríguez, Lucas José
dc.date.accessioned2023-06-18T06:57:31Z
dc.date.available2023-06-18T06:57:31Z
dc.date.issued2016
dc.description.abstractLeishmaniasis, caused by Leishmania infantum, is a vector-borne zoonotic disease that is endemic to the Mediterranean basin. The potential of rabbits and hares to serve as competent reservoirs for the disease has recently been demonstrated, although assessment of the importance of their role on disease dynamics is hampered by the absence of quantitative knowledge on the accuracy of diagnostic techniques in these species. A Bayesian latent-class model was used here to estimate the sensitivity and specificity of the Immuno-fluorescence antibody test (IFAT) in serum and a Leishmania-nested PCR (Ln-PCR) in skin for samples collected from 217 rabbits and 70 hares from two different populations in the region of Madrid, Spain. A two-population model, assuming conditional independence between test results and incorporating prior information on the performance of the tests in other animal species obtained from the literature, was used. Two alternative cut-off values were assumed for the interpretation of the IFAT results: 1/50 for conservative and 1/25 for sensitive interpretation. Results suggest that sensitivity and specificity of the IFAT were around 70–80%, whereas the Ln-PCR was highly specific (96%) but had a limited sensitivity (28.9% applying the conservative interpretation and 21.3% with the sensitive one). Prevalence was higher in the rabbit population (50.5% and 72.6%, for the conservative and sensitive interpretation, respectively) than in hares (6.7% and 13.2%). Our results demonstrate that the IFAT may be a useful screening tool for diagnosis of leishmaniasis in rabbits and hares. These results will help to design and implement surveillance programmes in wild species, with the ultimate objective of early detecting and preventing incursions of the disease into domestic and human populations.
dc.description.facultyCentro de Vigilancia Sanitaria Veterinaria (VISAVET)
dc.description.refereedTRUE
dc.description.sponsorshipUnión Europea. FP7
dc.description.sponsorshipComunidad de Madrid
dc.description.statusinpress
dc.eprint.idhttps://eprints.ucm.es/id/eprint/39703
dc.identifier.doi10.1002/vms3.37
dc.identifier.issn20531095
dc.identifier.officialurlhttp://dx.doi.org/10.1002/vms3.37
dc.identifier.urihttps://hdl.handle.net/20.500.14352/24670
dc.issue.number3
dc.journal.titleVeterinary Medicine and Science
dc.language.isoeng
dc.page.final220
dc.page.initial211
dc.publisherJohn Wiley & Sons
dc.relation.projectIDANTIGONE (278976)
dc.relation.projectIDTAVS (S2013/ABI-2747)
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/es/
dc.subject.keywordLeishmania infantum
dc.subject.keywordLagomorphs
dc.subject.keywordDiagnosis
dc.subject.keywordIFAT
dc.subject.keywordLn-PCR
dc.subject.keywordBayesian modelling
dc.subject.ucmVeterinaria
dc.subject.unesco3109 Ciencias Veterinarias
dc.titleAssessment of the sensitivity and specificity of serological (IFAT) and molecular (direct PCR) techniques for diagnosis of leishmaniasis in lagomorphs using a Bayesian approach
dc.typejournal article
dc.volume.number2
dspace.entity.typePublication
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relation.isAuthorOfPublication5fdbd68c-ea21-4625-ae57-296ae7183a64
relation.isAuthorOfPublication04121ef5-d450-407c-ade5-a0cbab05cb04
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relation.isAuthorOfPublication.latestForDiscovery08938f4f-d4f0-4b9c-a065-2df7e0b21f82
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