Publication:
Low abundance of NDUFV2 and NDUFS4 subunits of the hydrophilic complex I domain and VDAC1 predicts mammalian longevity

dc.contributor.authorMota-Martorell, Natalia
dc.contributor.authorJové, Mariona
dc.contributor.authorPradas, Irene
dc.contributor.authorSánchez, Isabel
dc.contributor.authorGómez, José
dc.contributor.authorNaudí, Alba
dc.contributor.authorBarja de Quiroga, Gustavo
dc.contributor.authorPamplona, Reinald
dc.date.accessioned2023-06-16T15:19:39Z
dc.date.available2023-06-16T15:19:39Z
dc.date.issued2020-04-20
dc.description.abstractMitochondrial reactive oxygen species (ROS) production, specifically at complex I (Cx I), has been widely suggested to be one of the determinants of species longevity. The present study follows a comparative approach to analyse complex I in heart tissue from 8 mammalian species with a longevity ranging from 3.5 to 46 years. Gene expression and protein content of selected Cx I subunits were analysed using droplet digital PCR (ddPCR) and western blot, respectively. Our results demonstrate: 1) the existence of species-specific differences in gene expression and protein content of Cx I in relation to longevity; 2) the achievement of a longevity phenotype is associated with low protein abundance of subunits NDUFV2 and NDUFS4 from the matrix hydrophilic domain of Cx I; and 3) long-lived mammals show also lower levels of VDAC (voltage-dependent anion channel) amount. These differences could be associated with the lower mitochondrial ROS production and slower aging rate of long-lived animals and, unexpectedly, with a low content of the mitochondrial permeability transition pore in these species.
dc.description.departmentDepto. de Genética, Fisiología y Microbiología
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y Competitividad (MINECO). Instituto de Salud Carlos III
dc.description.sponsorshipMinisterio de Ciencia, Innovación y Universidades (MICIU)
dc.description.sponsorshipGeneralitat de Cataluña
dc.description.sponsorshipFondo Europeo de Desarrollo Regional(FEDER)
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/61317
dc.identifier.doi10.1016/j.redox.2020.101539
dc.identifier.issn2213-2317, ESSN: 2213-2317
dc.identifier.officialurlhttps://www.sciencedirect.com/science/article/pii/S2213231720303281
dc.identifier.urihttps://hdl.handle.net/20.500.14352/6362
dc.issue.number101539
dc.journal.titleRedox Biology
dc.language.isoeng
dc.page.final20
dc.page.initial1
dc.publisherElsevier
dc.relation.projectID(PI14/00328)
dc.relation.projectID(RTI2018-099200-B-I00)
dc.relation.projectID(2017SGR696), SLT002/16/00250, PR [19] BIO MET 0155,AGAUR, ref 2018FI_B2_00104)
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subject.cdu591.1
dc.subject.cdu577.112
dc.subject.cdu599
dc.subject.keywordComplex I
dc.subject.keywordDroplet digital PCR
dc.subject.keywordLongevity
dc.subject.keywordMammals
dc.subject.keywordMitochondria
dc.subject.keywordNDUFV2 subunit
dc.subject.keywordNDUFS4 subunit
dc.subject.keywordVDAC
dc.subject.keywordWestern blot
dc.subject.ucmBioquímica (Biología)
dc.subject.ucmFisiología animal (Biología)
dc.subject.ucmMamíferos
dc.subject.unesco2302 Bioquímica
dc.subject.unesco2401.13 Fisiología Animal
dc.subject.unesco2401.18 Mamíferos
dc.titleLow abundance of NDUFV2 and NDUFS4 subunits of the hydrophilic complex I domain and VDAC1 predicts mammalian longevity
dc.typejournal article
dc.volume.number34
dspace.entity.typePublication
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