Polymerase chain reaction detection of avipox and avian papillomavirus in naturally infected wild birds: comparisons of blood, swab and tissue samples

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dc.contributor.authorWilliams, Richard Alexander John
dc.contributor.authorEscudero Duch, Clara
dc.contributor.authorPérez Tris, Javier
dc.contributor.authorBenítez Rico, Laura
dc.date.accessioned2024-02-07T09:18:30Z
dc.date.available2024-02-07T09:18:30Z
dc.date.issued2014-02-13
dc.descriptionThis research was funded by the Ministerio de Ciencia e Innovación, Spain (grant number: CGL2010-15734/BOS)
dc.description.abstractAvian poxvirus (avipox) is widely reported from avian species, causing cutaneous or mucosal lesions. Mortality rates of up to 100% are recorded in some hosts. Three major avipox clades are recognized. Several diagnostic techniques have been reported, with molecular techniques used only recently. Avipox has been reported from 278 different avian species, but only 111 of these involved sequence and/or strain identification. Collecting samples from wild birds is challenging as only few wild bird individuals or species may be symptomatic. Also, sampling regimes are tightly regulated and the most efficient sampling method, whole bird collection, is ethically challenging. In this study, three alternative sampling techniques (blood, cutaneous swabs and tissue biopsies) from symptomatic wild birds were examined. Polymerase chain reaction was used to detect avipoxvirus and avian papillomavirus (which also induces cutaneous lesions in birds). Four out of 14 tissue samples were positive but all 29 blood samples and 22 swab samples were negative for papillomavirus. All 29 blood samples were negative but 6/22 swabs and 9/14 tissue samples were avipox-positive. The difference between the numbers of positives generated from tissue samples and from swabs was not significant. The difference in the avipox-positive specimens in paired swab (4/6) and tissue samples (6/6) was also not significant. These results therefore do not show the superiority of swab or tissue samples over each other. However, both swab (6/22) and tissue (8/9) samples yielded significantly more avipox-positive cases than blood samples, which are therefore not recommended for sampling these viruses.
dc.description.departmentDepto. de Genética, Fisiología y Microbiología
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.statuspub
dc.identifier.citationWilliams RAJ, Escudero Duch C, Pérez-Tris J, Benítez L. Polymerase chain reaction detection of avipox and avian papillomavirus in naturally infected wild birds: comparisons of blood, swab and tissue samples. Avian pathology. 2014 Mar 4;43(2):130-4.
dc.identifier.doi10.1080/03079457.2014.886326
dc.identifier.issn0307-9457
dc.identifier.officialurlhttps://www.tandfonline.com/doi/full/10.1080/03079457.2014.886326
dc.identifier.urihttps://hdl.handle.net/20.500.14352/99801
dc.journal.titleAvian Pathology
dc.language.isoeng
dc.page.final134
dc.page.initial130
dc.publisherTaylor & Francis Online
dc.relation.projectIDCGL2010-15734/BOS
dc.rights.accessRightsrestricted access
dc.subject.cdu598.2
dc.subject.ucmAves
dc.subject.ucmMicrobiología (Biología)
dc.subject.ucmMedio ambiente natural
dc.subject.unesco2401 Biología Animal (Zoología)
dc.titlePolymerase chain reaction detection of avipox and avian papillomavirus in naturally infected wild birds: comparisons of blood, swab and tissue samples
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number4
dspace.entity.typePublication
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relation.isAuthorOfPublication7153d770-6b8a-45ce-babb-dc6d3c923fa8
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relation.isAuthorOfPublication.latestForDiscoveryfbe74799-7e79-4575-8569-13513d454d3e
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