Sequence determination and molecular characterization of gigantin, a cytotoxic protein produced by the mouldAspergillus giganteusIFO 5818

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dc.contributor.authorJérémie Wirth
dc.contributor.authorMartínez Del Pozo, Álvaro
dc.contributor.authorMancheño Gómez, José Miguel
dc.contributor.authorMartínez Ruiz, Antonio
dc.contributor.authorLacadena García-Gallo, Francisco Javier
dc.contributor.authorOñaderra Sánchez, Mercedes
dc.contributor.authorGavilanes Franco, José Gregorio
dc.date.accessioned2024-04-03T14:09:24Z
dc.date.available2024-04-03T14:09:24Z
dc.date.issued1997-07
dc.description.abstractGigantin is a 17-kDa ribonuclease secreted by Aspergillus giganteus IFO 5818. The sequence of the genomic DNA coding for this protein is reported. The deduced amino acid sequence reveals nine amino acid variations with respect to alpha-sarcin, a well-characterized ribosome-inactivating protein from A. giganteus MDH 18894. The peptides obtained after tryptic digestion of reduced and carboxyamidomethylated gigantin have been chromatographically separated. The analysis of these peptides in comparison to those originating from alpha-sarcin corroborates the above sequence differences. These do not sensibly modify the conformation of the protein, based on the coincidence of the circular dichroism and fluorescence emission spectra of the two proteins. The obtained results are discussed in terms of the involvement of the distinctive residues in the immunological and catalytic properties that distinguish gigantin from alpha-sarcin.
dc.description.departmentDepto. de Bioquímica y Biología Molecular
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.statuspub
dc.identifier.citationArch Biochem Biophys . 1997 Jul 15;343(2):188-93
dc.identifier.doi10.1006/abbi.1997.0175
dc.identifier.issn0003-9861
dc.identifier.urihttps://hdl.handle.net/20.500.14352/102625
dc.issue.number2
dc.journal.titleArchives of Biochemestry and Biophysics
dc.language.isoeng
dc.page.final193
dc.page.initial188
dc.rights.accessRightsopen access
dc.subject.cdu577.1
dc.subject.keywordAspergillus ribotoxin
dc.subject.keywordRibosome inactivating protein
dc.subject.keywordα-sarcin
dc.subject.ucmBioquímica (Química)
dc.subject.unesco2302 Bioquímica
dc.titleSequence determination and molecular characterization of gigantin, a cytotoxic protein produced by the mouldAspergillus giganteusIFO 5818
dc.typejournal article
dc.volume.number343
dspace.entity.typePublication
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