Influence of Mesenchymal Stem Cell Sources on Their Regenerative Capacities on Different Surfaces

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dc.contributor.authorMucientes, Arkaitz
dc.contributor.authorHerranz, Eva
dc.contributor.authorMoro Rodríguez, Luis Enrique
dc.contributor.authorGonzález Corchón, Aranzazu
dc.contributor.authorPeña-Soria, María Jesús
dc.contributor.authorAbásolo Alcázar, Lydia
dc.contributor.authorRodriguez Rodriguez, Luis
dc.contributor.authorLamas, Jose Ramon
dc.contributor.authorFernández Gutiérrez, Benjamín
dc.date.accessioned2023-06-17T08:32:11Z
dc.date.available2023-06-17T08:32:11Z
dc.date.issued2021
dc.description.abstractCurrent gold-standard strategies for bone regeneration do not achieve the optimal recovery of bone biomechanical properties. To bypass these limitations, tissue engineering techniques based on hybrid materials made up of osteoprogenitor cells—such as mesenchymal stem cells (MSCs)—and bioactive ceramic scaffolds—such as calcium phosphate-based (CaPs) bioceramics—seem promising. The biological properties of MSCs are influenced by the tissue source. This study aims to define the optimal MSC source and construct (i.e., the MSC–CaP combination) for clinical application in bone regeneration. A previous iTRAQ analysis generated the hypothesis that anatomical proximity to bone has a direct effect on MSC phenotype. MSCs were isolated from adipose tissue, bone marrow, and dental pulp, then cultured both on a plastic surface and on CaPs (hydroxyapatite and β-tricalcium phosphate), to compare their biological features. On plastic, MSCs isolated from dental pulp (DPSCs) presented the highest proliferation capacity and the greatest osteogenic potential. On both CaPs, DPSCs demonstrated the greatest capacity to colonise the bioceramics. Furthermore, the results demonstrated a trend that DPSCs had the most robust increase in ALP activity. Regarding CaPs, β-tricalcium phosphate obtained the best viability results, while hydroxyapatite had the highest ALP activity values. Therefore, we propose DPSCs as suitable MSCs for cell-based bone regeneration strategies.
dc.description.departmentDepto. de Cirugía
dc.description.facultyFac. de Medicina
dc.description.refereedTRUE
dc.description.sponsorshipInstituto de Salud Carlos III
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/77453
dc.identifier.doi10.3390/cells10020481
dc.identifier.issn2073-4409
dc.identifier.officialurlhttps://doi.org/10.3390/cells10020481
dc.identifier.relatedurlhttps://www.mdpi.com/2073-4409/10/2/481
dc.identifier.urihttps://hdl.handle.net/20.500.14352/7388
dc.issue.number2
dc.journal.titleCells
dc.language.isoeng
dc.page.initial481
dc.publisherMDPI
dc.relation.projectIDRIER ; FIS PI13/01863
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/es/
dc.subject.cdu575
dc.subject.keywordMesenchymal stem cell
dc.subject.keywordDental pulp
dc.subject.keywordBeta-tricalcium phosphate
dc.subject.keywordHydroxyapatite
dc.subject.keywordOsteogenesis
dc.subject.keywordRegenerative medicine
dc.subject.ucmMedicina
dc.subject.ucmCirugía
dc.subject.ucmBiología celular (Biología)
dc.subject.ucmGenética
dc.subject.unesco32 Ciencias Médicas
dc.subject.unesco3213 Cirugía
dc.subject.unesco2407 Biología Celular
dc.subject.unesco2409 Genética
dc.titleInfluence of Mesenchymal Stem Cell Sources on Their Regenerative Capacities on Different Surfaces
dc.typejournal article
dc.volume.number10
dspace.entity.typePublication
relation.isAuthorOfPublicationfcb8449b-8936-4130-a701-3584412a6480
relation.isAuthorOfPublicationa39703bb-180c-4f76-a2ef-be65b6515a5d
relation.isAuthorOfPublication.latestForDiscoveryfcb8449b-8936-4130-a701-3584412a6480
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