Fast Electrochemical miRNAs Determination in Cancer Cells and Tumor Tissues with Antibody-Functionalized Magnetic Microcarriers

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dc.contributor.authorTorrente Rodríguez, Rebeca Magnolia
dc.contributor.authorRuiz Valdepeñas Montiel, Víctor
dc.contributor.authorCampuzano Ruiz, Susana
dc.contributor.authorFarchado-Dinia, Meryem
dc.contributor.authorBarderas Manchado, Rodrigo
dc.contributor.authorSan Segundo Acosta, Pablo
dc.contributor.authorMontoya, Juan
dc.contributor.authorPingarrón Carrazón, José Manuel
dc.date.accessioned2024-01-25T12:57:49Z
dc.date.available2024-01-25T12:57:49Z
dc.date.issued2016
dc.descriptionLa metodología desarrollada en esta investigación fue patentada. A continuación se aportan datos relacionados con la misma: Título: Magnetic beads-based electrochemical biosensor Pub . No . : US 2019 / 0048402 A1 Pub . Date : Feb . 14 , 2019
dc.description.abstractMicroribonucleic acids (miRNAs) have been linked with various regulatory functions and diseases and constitute important targets in future medical diagnostics and prognostics. We report here a novel sensitive and rapid bioelectrochemical strategy for miRNA determination. This strategy involves the development of a sensing approach making use of magnetic beads (MBs) modified with a specific DNA-RNA antibody as capture bioreceptor and amperometric detection implying the H2O2/hydroquinone (HQ) system at disposable screen-printed carbon electrodes (SPCEs). The developed biosensor exhibits a dynamic range from 8.2 to 250 pM and a detection limit of 2.4 pM (60 amol) of a synthetic target without any amplification step in 2 h. The usefulness of the approach was evaluated by analyzing total RNA (RNAt) extracted from metastatic cancer cell lines and human tumor tissues, which demonstrated its potential to perform determination of mature miRNAs in these complex samples. Moreover, the feasibility of the developed methodology to detect simultaneously the expression of two different miRNAs at dual SPCEs (SPdCEs) in one single experiment was also explored. The feasibility to capture and release target miRNAs make the developed methodology also an attractive tool to isolate, purify, and determine target miRNAs with great applicability in the clinical field.
dc.description.departmentDepto. de Química Analítica
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y Competitividad (España)
dc.description.sponsorshipComunidad de Madrid
dc.description.sponsorshipMinisterio de Educación (España)
dc.description.statuspub
dc.identifier.citationRebeca M. Torrente-Rodríguez, Víctor Ruiz-Valdepeñas Montiel, Susana Campuzano, Meryem Farchado-Dinia, Rodrigo Barderas, Pablo San Segundo-Acosta, Juan J. Montoya, and José M. Pingarron ACS Sensors 2016 1 (7), 896-903 DOI: 10.1021/acssensors.6b00266
dc.identifier.doi10.1021/acssensors.6b00266
dc.identifier.issn2379-3694
dc.identifier.officialurlhttps://doi.org/10.1021/acssensors.6b00266
dc.identifier.urihttps://hdl.handle.net/20.500.14352/95446
dc.journal.titleACS Sensors
dc.language.isoeng
dc.page.final903
dc.page.initial896
dc.publisherAmerican Chemical Society
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO//CTQ2015-64402-C2-1-R/ES/NUEVAS PLATAFORMAS DE MULTIPLEXADO ELECTROANALITICAS PARA LA DETECCION Y PRONOSTICO DE ENFERMEDADES NEOPLASICAS MEDIANTE BIOPSIAS LIQUIDAS/
dc.relation.projectIDS2013/MT−3029
dc.rights.accessRightsrestricted access
dc.subject.cdu543
dc.subject.keywordMiRNA
dc.subject.keywordDNA-RNA antibody
dc.subject.keywordMagnetic beads
dc.subject.keywordSPCEs
dc.subject.keywordDual detection
dc.subject.keywordCancer cell
dc.subject.keywordTumor tissues
dc.subject.ucmQuímica analítica (Química)
dc.subject.unesco2301 Química Analítica
dc.titleFast Electrochemical miRNAs Determination in Cancer Cells and Tumor Tissues with Antibody-Functionalized Magnetic Microcarriers
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number1
dspace.entity.typePublication
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