Degradation of Human Serum Albumin by Infrared Free Electron Laser Enhanced by Inclusion of a Salen-Type Schiff Base Zn (II) Complex

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dc.contributor.authorOnami, Yuika
dc.contributor.authorKawasaki, Takayasu
dc.contributor.authorAizawa, Hiroki
dc.contributor.authorHaraguchi, Tomoyuki
dc.contributor.authorAkitsu, Takashiro
dc.contributor.authorTsukiyama, Koichi
dc.contributor.authorAlcolea Palafox, Mauricio
dc.date.accessioned2023-06-17T09:08:12Z
dc.date.available2023-06-17T09:08:12Z
dc.date.issued2020-01-29
dc.description.abstractA salen-type Schiff base Zn(II) complex included in human serum albumin (HSA) protein was examined by UV-Vis, circular dichroism (CD), and fluorescence (PL) spectra. The formation of the composite material was also estimated by a GOLD program of ligand–protein docking simulation. A composite cast film of HSA and Zn(II) complex was prepared, and the effects of the docking of the metal complex on the degradation of protein molecules by mid-infrared free electron laser (IR-FEL) were investigated. The optimum wavelengths of IR-FEL irradiation to be used were based on experimental FT-IR spectra and vibrational analysis. Using TD-DFT results with 6-31G(d,p) and B3LYP, the IR spectrum of Zn(II) complex could be reasonably assigned. The respective wavelengths were 1652 cm−1 (HSA amide I), 1537 cm−1 (HSA amide II), and 1622 cm−1 (Zn(II) complex C=N). Degradation of HSA based on FT-IR microscope (IRM) analysis and protein secondary structure analysis program (IR-SSE) revealed that the composite material was degraded more than pure HSA or Zn(II) complex; the inclusion of Zn(II) complex enhanced destabilization of folding of HSA.
dc.description.departmentDepto. de Química Física
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/66279
dc.identifier.doi10.3390/ijms21030874
dc.identifier.issn1422-0067
dc.identifier.officialurlhttps://doi.org/10.3390/ijms21030874
dc.identifier.relatedurlhttps://www.mdpi.com/1422-0067/21/3/874
dc.identifier.urihttps://hdl.handle.net/20.500.14352/8250
dc.issue.number3
dc.journal.titleInternational Journal of Molecular Sciences
dc.language.isoeng
dc.page.initial874
dc.publisherMDPI
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/es/
dc.subject.keywordIR-FEL
dc.subject.keywordhuman serum albumin
dc.subject.keywordSchiff base
dc.subject.keywordZn(II) complex
dc.subject.keywordTD-DFT
dc.subject.keywordfluorescence
dc.subject.ucmQuímica física (Química)
dc.titleDegradation of Human Serum Albumin by Infrared Free Electron Laser Enhanced by Inclusion of a Salen-Type Schiff Base Zn (II) Complex
dc.typejournal article
dc.volume.number21
dspace.entity.typePublication
relation.isAuthorOfPublication1163c7a4-e779-417f-867c-a6e963e4525e
relation.isAuthorOfPublication.latestForDiscovery1163c7a4-e779-417f-867c-a6e963e4525e
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