Dynamics of sperm DNA fragmentation in domestic animals: II. The stallion

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dc.contributor.authorLopez-Fernandez, C.
dc.contributor.authorCrespo Castejón, Francisco
dc.contributor.authorArroyo, F.
dc.contributor.authorFernandez, J. L.
dc.contributor.authorArana Montes, María Del Pilar De
dc.contributor.authorJohnston, S. D.
dc.contributor.authorGosalvez, J.
dc.date.accessioned2024-01-17T09:18:37Z
dc.date.available2024-01-17T09:18:37Z
dc.date.issued2007-10-04
dc.description.abstractThe mixed success of equine artificial insemination programs using chilled and frozen-thawed semen is most likely associated with the variable response of the sperm cell to the preservation process and the fact that stallions are not selected on the basis of reproductive performance. We propose that the traditional indicators of sperm viability do not fully account for male factor infertility in the stallion and that knowledge of sperm DNA damage in the original semen sample and during semen processing may provide a more informed explanation of an individual stallion's reproductive potential. This study reports on the validation of a sperm DNA fragmentation test based on the sperm chromatin dispersion test (SCD) for stallion spermatozoa and on its application to semen that was chilled (4 °C; n = 10) or frozen-thawed (n = 13). Semen samples were collected by artificial vagina and the proportion of sperm with fragmented DNA determined. Seminal plasma was then removed by centrifugation and the sperm pellet re-suspended in commercial extenders prior to being chilled or cryopreserved using standard industry protocols. Chilled semen was cooled slowly to 4 °C and stored for 1 h before commencing the analysis; cryopreserved semen was thawed and immediately analyzed. Following chilling or cryopreservation, the semen samples were incubated at 37 °C and analyzed for SCD after 0, 4, 6, 24 and 48 h storage. The results of this investigation revealed that there was no significant difference in the sperm DNA fragmentation index (sDFI) of sperm evaluated initially after collection compared to those tested immediately after chilling or cryopreservation. However, within 1 h of incubation at 37 °C, both chilled and frozen-thawed spermatozoa showed a significant increase in the proportion of sDFI; after 6 h the sDFI had increased to over 50% and by 48 h, almost 100% of the sperm showed DNA damage. While the sDFI of individual stallions at equivalent times of incubation was variable, an analysis of the rate of change of sDFI revealed no difference between stallions or the way in which the semen was preserved. In terms of sperm DNA fragmentation dynamics, the highest intensity of sperm DNA damage occurred in the first 6 h of incubation. We suggest that the SCD test can be used as a routine assessment tool for the development and refinement of preservation protocols designed to reduce stallion sperm DNA damage.
dc.description.departmentDepto. de Genética, Fisiología y Microbiología
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Educación y Ciencia
dc.description.statuspub
dc.identifier.citationLópez-Fernández, C., Crespo, F., Arroyo, F., Fernández, J. L., Arana, P., Johnston, S. D., & Gosálvez, J. (2007). Dynamics of sperm DNA fragmentation in domestic animals: II. The stallion. Theriogenology, 68(9), 1240-1250.
dc.identifier.doi10.1016/j.theriogenology.2007.08.029
dc.identifier.essn1879-3231
dc.identifier.issn0093-691X
dc.identifier.officialurlhttps://www.sciencedirect.com/science/article/pii/S0093691X07005079
dc.identifier.urihttps://hdl.handle.net/20.500.14352/93530
dc.issue.number9
dc.journal.titleTheriogenology
dc.language.isoeng
dc.page.final1250
dc.page.initial1240
dc.publisherElsevier BV
dc.relation.projectID(MEC-BFU-2007-66340)
dc.rights.accessRightsrestricted access
dc.subject.cdu636.082.4
dc.subject.cdu636.06
dc.subject.cdu575.827
dc.subject.keywordAndrology
dc.subject.keywordSperm
dc.subject.keywordSperm DNA fragmentation
dc.subject.keywordStallion
dc.subject.keywordSCD
dc.subject.ucmGenética
dc.subject.ucmMamíferos
dc.subject.unesco2401.08 Genética Animal
dc.titleDynamics of sperm DNA fragmentation in domestic animals: II. The stallion
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number68
dspace.entity.typePublication
relation.isAuthorOfPublicationd4b46bbe-e6cb-410e-8b74-b2361b41941d
relation.isAuthorOfPublicationf751ab61-9b93-48a2-9c20-6438cd33c2c4
relation.isAuthorOfPublication.latestForDiscoveryf751ab61-9b93-48a2-9c20-6438cd33c2c4
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