No seed size–number trade‐off in European beech: climate governs investment per seed

Abstract

Mast-seeding trees can vary seed output by orders of magnitude among years, but it remains unclear whether high seed production reduces per-seed investment, as predicted by fixed-budget allocation models. We quantified individual seed production with seed mass in European beech across 2792 trees and 123 populations spanning the species' range and quantified seed protein and lipid content in 35 populations. Seed mass increased with seed production, with seeds from high-seeding years being 14% heavier than those from low-seeding years, providing no evidence for a seed size–number trade-off and instead supporting variable reproductive allocation. By contrast, protein content decreased by 31% with increasing seed production, whereas lipid content increased (by 14%), indicating that nitrogen becomes constraining at high reproductive output while carbon-based provisioning is maintained. Climate further structured provisioning: seed mass and protein content were the lowest at climatic range margins, being 28% and 32% lower, respectively, than at the center of the climatic range. European beech can increase seed output without reducing per-seed biomass, but that nitrogen limitation and climatic constraints may strengthen regeneration bottlenecks at both trailing and leading margins, especially as climate warming intensifies.