Cholesterol stimulates and ceramide inhibits Sticholysin II-induced pore formation in complex bilayer membranes

Simple item page
dc.contributor.authorAlm, Ida
dc.contributor.authorGarcía Linares, Sara
dc.contributor.authorGavilanes, José G.
dc.contributor.authorMartínez del Pozo, Álvaro
dc.contributor.authorSlotte, J. Peter
dc.date.accessioned2023-06-18T05:48:32Z
dc.date.available2023-06-18T05:48:32Z
dc.date.issued2015-04
dc.description.abstractThe pore forming capacity of Sticholysin II (StnII; isolated from Stichodactyla helianthus) in bilayer membranes containing 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), palmitoylsphingomyelin (PSM) and either cholesterol or palmitoyl ceramide (PCer) has been examined. The aim of the study was to elucidate how the presence of differently ordered PSM domains affected StnII oligomerization and pore formation. Cholesterol is known to enhance pore formation by StnII, and our results confirmed this and provide kinetic information for the process. The effect of cholesterol on bilayer permeabilization kinetics was concentration-dependent. In the concentration regime used (2.5–10 nmol cholesterol in POPC:PSM 80:20 by nmol), cholesterol also increased the acyl chain order in the fluid PSM domain and thus decreased bilayer fluidity, suggesting that fluidity per se was not responsible for cholesterol's effect. Addition of PCer (2.5–10 nmol) to the POPC:PSM (80:20 by nmol) bilayers attenuated StnII-induced pore formation, again in a concentration-dependent fashion. This addition also led to the formation of a PCer-rich gel phase. Addition of cholesterol to PCer-containing membranes could partially reduce the inhibitory effect of PCer on StnII pore formation. We conclude that the physical state of PSM (as influenced by either cholesterol or PCer) affected StnII binding and pore formation under the conditions examined.
dc.description.departmentSección Deptal. de Bioquímica y Biología Molecular (Biológicas)
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia e Innovación (MICINN)
dc.description.sponsorshipSigrid Juselius Foundation
dc.description.sponsorshipÅbo Akademi Foundation
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/43469
dc.identifier.doi10.1016/j.bbamem.2014.12.017
dc.identifier.issn0006-3002
dc.identifier.officialurlhttps://www.sciencedirect.com/science/article/pii/S0005273614004532
dc.identifier.urihttps://hdl.handle.net/20.500.14352/23386
dc.issue.number4
dc.journal.titleBBA - Biochimica et Biophysica Acta
dc.language.isoeng
dc.page.final931
dc.page.initial925
dc.publisherElsevier
dc.relation.projectIDBFU2012-32404
dc.rights.accessRightsrestricted access
dc.subject.cdu577.2
dc.subject.keywordMembrane permeabilization
dc.subject.keywordSurface plasmon resonance
dc.subject.keywordSphingolipid
dc.subject.keywordMembrane order
dc.subject.ucmBiología
dc.subject.ucmBiología molecular (Biología)
dc.subject.unesco24 Ciencias de la Vida
dc.subject.unesco2415 Biología Molecular
dc.titleCholesterol stimulates and ceramide inhibits Sticholysin II-induced pore formation in complex bilayer membranes
dc.typejournal article
dc.volume.number1848
dspace.entity.typePublication
relation.isAuthorOfPublication35824f7f-c79d-4928-9728-21124243bf7a
relation.isAuthorOfPublication.latestForDiscovery35824f7f-c79d-4928-9728-21124243bf7a
Download
Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Gavilanes. 2015. Cholesterol stimulates and ceramide inhibits Sticholysin II-induced pore formation in complex bilayer membranes.pdf
Size:
1.01 MB
Format:
Adobe Portable Document Format
Download
Collections
Artículos