Dielectric characterization of bacterial cells using dielectrophoresis

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Measurements of dielectrophoretic collection spectra of Escherichia coli and Staphylococcus aureus suspensions are used for obtaining dielectric characteristics of both types of bacteria. The experiments are interpreted using a numerical method that models the cells as compartmented spherical or rod-like particles. We show the usefulness of this simple method to extract significant information about the electrical properties of Gram-negative and -positive bacteria.
© Wiley-Liss, Inc. Financial support from a EU grant (QLK2-CT-2001-70561 RASTUD) is gratefully acknowledged.
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Asami K, Hanai KT, Koizumi N. 1980a. Dielectric approach to suspensions of ellipsoidal particles covered with a shell in particular reference to biological cells. Jpn J Appl Phys 19:359–365. Asami K, Hanai KT, Koizumi N. 1980b. Dielectric analysis of Escherichia coli suspensions in the light of the theory of interfacial polarization. Biophys J 31:215–228. Becker FF, Wang XB, Huang Y, Pethig R, Vykoukal J, Gascoyne PRC. 1995. Separation of human breast cancer cells from blood by differential dielectric affinity. Proc Natl Acad Sci USA 92:860–864. Broche LM, Labeed FH, Hughes MP. 2005. Extraction of dielectric properties of multiple populations from dielectrophoretic collection spectrum data. Phys Med Biol 50:2267–2274. Brown AP, Betts WB, Harrison AB, O’Neill JG. 1999. Evaluation of a dielectrophoretic bacterial counting technique. Biosens Bioelectron 14:341–35. Burt JPH, Pethig R, Gascoyne PRC, Becker FF. 1990. Dielectrophoretic characterisation of Friend murine erythroleukaemic cells as a measure of induced differentiation. Biochim Biophys 1034:93–101. Docoslis A, Kalogerakis N, Behie LA. 1999. Dielectrophoretic forces can be safely used to retain viable cells in perfusion cultures of animal cells. Cytotechnology 30:133–142. Foster KR, Sowers AE. 1995. Dielectrophoretic forces and potentials induced on pairs of cells in an electric field. Biophys J 69:777–784. Foster KR, Sauer FA, Schwan HP. 1992. Electrorotation of cells and colloidal particles. Biophys J 63:180–190. Fricke H. 1925. A mathematical treatment of the electric conductivity and capacity of disperse systems II. The capacity of a suspension of conducting spheroids surrounded by a non-conducting membrane for a current of low frequency. Phys Rev 26:678–681. Fuhr G, Gimsa J, Glaser R. 1985. Interpretation of electrorotation of protoplast. Stud Biophys 108:149–164. Gimsa J. 2001. Characterization of particles and biological cells by AC electrokinetics. In: Delgado AV, editor. Interfacial electrokinetics and electrophoresis. New York: Marcel Dekker Inc. pp 369–400. Gimsa J, Schnelle T, Zechel G, Glaser R. 1994. Dielectric spectroscopy of human erythrocytes: investigation under the influence of nystatin. Biophys J 66:1244–1253. Hecker M, Engelmann S, Cordwell SJ. 2003. Proteomics of Staphylococcus aureus—current state and future challenges. J Chromatogr B 787(1):179–195. Hinds WC. 1999. Aerosol Technology: Properties, Behavior, and Measurement of Airborne Particles. New York: Wiley & Sons. Hözel R. 2002. Single particles characterization and manipulation by opposite field dielectrophoresis. J Electrostat 56:435–447. Huang Y, Hözel R, Pethig R, Wang X-B. 1992. Differences in the AC electrodynamics of viable and no viable yeast cells determined trough combined dielectrophoresis and electrorotation studies. Phys Med Biol 37:1499–1517. Hughes MP, Morgan H, Rixon FJ. 2002. Measuring the dielectric properties of herpes simplex virus type 1 virions with dielectrophoresis. Biochim Biophys Acta 1571:1 8. Ikeda I, Tsukahara S, Watarai H. 2003. Effects of viability and lectin protein binding on dielectrophoretic behavior of single yeast cells. Anal Sci 19:27–31. Johari J, Hübner Y, Hull JC, Dale JW, Hughes MP. 2003. Dielectrophoretic assay of bacterial resistance to antibiotics. Phys Med Biol 48:193–198. Labeed FH, Coley HM, Thomas H, Hughes PM. 2003. Assessment of multidrug resistance reserval using dielectrophoresis and flow cytometry. Biophys J 85:2028–2034. Lee SW, Tai YC. 1999. A micro cell lysis device. Sens Actuators A: Phys 73(1):74–79. Liang J, Subramaniam S. 1997. Computation of molecular electrostatics with boundary element methods. Biophys J 73:1830–1841. Miller RD, Jones TB. 1993. Electro-orientation of ellipsoidal erythrocytes. Theory and experiment. Biophys J 64:1588–1595. Milner KR, Brown AP, Allsopp DWE, Betts WB. 1998. Dielectrophoretic classification of bacteria using differential impedance measurements. IEE Electron Lett 34:66–68. Moat AG, Foster JW, Spector MP. 2002. Microbial Physiology. New York: Wiley-Liss. Morgan H, Green NG. 1997. Dielectrophoretic manipulation of rodshaped viral particles. J Electrostatistics 42:279–293. Nayfeh MH, Brussel MK. 1985. Electricity and Magnetism. New York: Wiley & Sons. Pethig R. 1979. Dielectric and Electronic Properties of Biological Materials. New York: Wiley & Sons. Pohl HA. 1978. Dielectrophoresis. Cambridge: Cambridge University Press. Quinn CM, Archer GP, Betts WB, O’Neill JG. 1995. Protozoan Parasites and Water. Betts WB et al., editors. Cambridge: Royal Society of Chemistry. pp. 125–132. Ratanachoo K, Gascoyne PRC, Ruchirawat M. 2002. Detection of cellular responses to toxicants by dielectrophoresis. Biochim Biophys Acta 1564:449–458. Sanchis A, Sancho M, Martínez G, Sebastián JL, Muñoz S. 2004. Interparticle forces in electrorheological fluids: effects of polydispersity and shape. Colloid Surf A 249:119 123. Sancho M, Martínez G, Martín C. 2003. Accurate dielectric modeling of shelled particles and cells. J Electrostat 57: 143–156. Suehiro J, Hamada R, Noutomi D, Shutou M, Hara M. 2003. Selective detection of viable bacteria using dielectrophoretic impedance measurement method. J Electrostat 57:157–168. Talary MS, Burt JPH, Tame JA, Pethig R. 1996. Electromanipulation and separation of cells using traveling electric fields. J Phys D: Appl Phys 29:2198–2203.