Docta Complutense
Open Access Institutional Repository of the Complutense University of Madrid, that compiles scientific production to promote the visibility and impact of Complutense research.
With the collaboration of the Ministerio de Ciencia e Innovación and the Spanish Foundation for Science and Technology (FECYT).
Recent Submissions
P2X7 receptor or TNAP genetic blockade prevents the reduction of neuronal CTCF detected in tauopathy
(Neuropharmacology, 2026) Aivar, Paloma; Sebastián Serrano, Álvaro; Vincelle-Nieto, África; Collado-Cantero, Ana C; Ouro-Corredera, Daniel; Soria Tobar, Lucía; Reyes Palomares, Armando Adolfo; Díaz Hernández, Miguel
Intraneuronal aggregates assembled by hyperphosphorylated Tau protein are a common feature of a neurodegenerative disease family, namely Tauopathies. Recent studies have revealed that the ionotropic purinergic receptor P2X7 (P2X7R) and the ectoenzyme tissue-nonspecific alkaline phosphatase (TNAP) are upregulated in patients with tauopathies. Despite the molecular mechanisms underlying their upregulation remaining unknown, several studies suggest that genetic or pharmacological blocking of both proteins may represent a promising therapeutic strategy. Here, we performed transcriptomic analyses of bulk RNA-seq data from human cortical samples and revealed altered activity of multiple transcription factors (TFs) in tauopathy patients. Among the most affected TFs, we focused on the chromatin organizer CCCTC-binding factor (CTCF), as it was the only one that bound both the P2X7R and TNAP promoters. Gene expression deconvolution analyses revealed altered CTCF expression in both neuronal and glial populations from tauopathy patients. Consistently, immunofluorescence analyses in P301S mice, a tauopathy mouse model, showed a significant reduction in neuronal CTCF expression and a substantial increase in the number of glial cells with CTCF labelling. In vitro studies using neuroblastoma N2a cells confirmed that CTCF overexpression caused a transcriptional upregulation of both P2X7R and TNAP. Moreover, the fact that P2X7R activation or TNAP blockade decreases CTCF nuclear translocation suggests a bidirectional regulation. In line with these findings, we found that selective genetic P2X7R depletion or, unexpectedly, TNAP heterozygosity prevented the reduction of neuronal CTCF expression in P301S mice. Altogether, these data suggest that the newly identified TNAP/P2X7R/CTCF signaling axis is a crucial molecular mechanism underlying tauopathies.
Todos los Santos: tradición y ayllu andino
(Chungará, 2017) Muñoz Morán, Óscar
RESUMEN: Los rituales dedicados a la muerte han ocupado siempre un lugar privilegiado dentro de la etnografía andina. Habitualmente analizados desde la funcionalidad o semiótica de los mismos, no ha sido habitual, como sí ha sucedido con otras prácticas regionales, encuadrarlas dentro de estructuras mayores. En este artículo me propongo analizar el momento actual de un ayllu quechua de Potosí, Bolivia, desde la lectura etnográfica (presente y pasada) de la fiesta de Todos los Santos. Esta festividad se presenta como el fiel reflejo del proceso de transformación intensa que está viviendo una estructura tan compleja como es el ayllu.
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