Person: Ortiz García, María Josefa
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First Name
María Josefa
Last Name
Ortiz García
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Químicas
Department
Química Orgánica
Area
Química Orgánica
Identifiers
7 results
Search Results
Now showing 1 - 7 of 7
- Project number: 77
Item Adaptación de las prácticas de laboratorio de Materiales en Óptica Oftálmica y Lentes de contacto a metodologías de aprendizaje activo basado en la experimentación(2023) Orden Hernández, María Ulagares De La; Escobar Peña, Ana Andrea; Jiménez García, Inmaculada; Lasagabaster Latorre, Aurora; Macicior Michelena, Jon; Martín-Fontecha Corrales, María Del Mar; Molina Santos, Marina Mercedes; Marinov, Lyuboslav Nikolaev; Ortiz García, María Josefa; Rodríguez Agarrabeitia, AntoniaEste proyecto pretende rediseñar las prácticas de Materiales Ópticos y adaptarlas a los principios del Aprendizaje Activo Basado en la Experimentación (ABE) implementando herramientas de Aula Invertida y Aprendizaje Cooperativo Item Red haloBODIPYs as theragnostic agents: The role of the substitution at meso position(Dyes and Pigments, 2021) Prieto Montero, Ruth; Prieto Castañeda, Alejandro; Katsumiti, Alberto; Sola Llano, Rebeca; Rodríguez Agarrabeitia, Antonia; Cajaraville, Miren P.; Ortiz García, María Josefa; Martínez Martínez, VirginiaThree different molecular designs based on BODIPY dye have been proposed as photosensitizers (PSs) for photodynamic therapy (PDT) by the inclusion of halogen atoms (Iodine) at 2,6-positions and with extended conjugation at 3, 5-positions and varying the substitution at meso position. The synthesis is described and their main photophysical features including singlet oxygen production and triplet states were characterized by absorption and fluorescence spectroscopy (steady-state and time-correlated) and nanosecond transient absorption spectroscopy. The results were compared with the commercial Chlorin e6. The three new red-halogen-BODIPYs showed a great balance between singlet oxygen generation (ΦΔ≥0.40) and fluorescence (Φfl≥0.22) for potential application on PDT, and particularly in theragnosis. In vitro experiments in HeLa cells were done to study their performance and to elucidate the best potential candidate for PDT.Item Development of Geometry-Controlled All-Orthogonal BODIPY Trimers for Photodynamic Therapy and Phototheragnosis(Organic Letters, 2022) Prieto Castañeda, Alejandro; García Garrido, Fernando; Díaz Norambuena, Carolina; Escriche Navarro, Blanca; García Fernández, Alba; Bañuelos, Jorge; Rebollar, Esther; García Moreno, Inmaculada; Martínez Máñez, Ramón; Moya Cerero, Santiago De La; Rodríguez Agarrabeitia, Antonia; Ortiz García, María JosefaWe have established an easy synthetic protocol for selectively developing all-orthogonal BODIPY trimers with unprecedented geometries on the basis of selecting methyl oxidation versus electrophilic formylation of key dimeric precursors. Photophysical characterization together with biological assays unraveled the most suitable BODIPY−BODIPY geometrical arrangements within the trimer, forcing them to serve as molecular platforms for the development of new, advanced heavy-atom-free photosensitizers for photodynamic therapy and phototheragnosis.Item Functionalization of photosensitized silica nanoparticles for advanced photodynamic therapy of cancer(International Journal of Molecular Sciences, 2021) Prieto Montero, Ruth; Prieto Castañeda, Alejandro; Katsumiti, Alberto; Cajaraville, Miren P.; Rodríguez Agarrabeitia, Antonia; Ortiz García, María Josefa; Martínez Martínez, VirginiaBODIPY dyes have recently attracted attention as potential photosensitizers. In this work, commercial and novel photosensitizers (PSs) based on BODIPY chromophores (haloBODIPYs and orthogonal dimers strategically designed with intense bands in the blue, green or red region of the visible spectra and high singlet oxygen production) were covalently linked to mesoporous silica nanoparticles (MSNs) further functionalized with PEG and folic acid (FA). MSNs approximately 50 nm in size with different functional groups were synthesized to allow multiple alternatives of PS-PEG-FA decoration of their external surface. Different combinations varying the type of PS (commercial Rose Bengal, Thionine and Chlorine e6 or custom-made BODIPY-based), the linkage design, and the length of PEG are detailed. All the nanosystems were physicochemically characterized (morphology, diameter, size distribution and PS loaded amount) and photophysically studied (absorption capacity, fluorescence efficiency, and singlet oxygen production) in suspension. For the most promising PS-PEG-FA silica nanoplatforms, the biocompatibility in dark conditions and the phototoxicity under suitable irradiation wavelengths (blue, green, or red) at regulated light doses (10–15 J/cm2) were compared with PSs free in solution in HeLa cells in vitro.Item From photosensitizers to light harvesters adapting the molecular structure in all-BODIPY assemblies(Physical chemistry chemical physics, 2021) Avellanal Zaballa, Edurne; Prieto Castañeda, Alejandro; Díaz Norambuena, Carolina; Bañuelos Prieto, Jorge; Rodríguez Agarrabeitia, Antonia; García Moreno, Inmaculada; Moya Cerero, Santiago de la; Ortiz García, María JosefaHerein we detail a protocol to design dyads and triads based solely on BODIPY dyes as halogen-free singlet oxygen photosensitizers or energy transfer molecular cassettes. The conducted photonic characterization reveals the key role of the BODIPY–BODIPY linkage to finely modulate the balance between the triplet state population and fluorescence decay.Item Phosphorogenic dipyrrinato-iridium(III) complexes as photosensitizers for photodynamic therapy(Phosphorogenic dipyrrinato-iridium(III) complexes as photosensitizers for photodynamic therapy, 2021) Prieto Castañeda, Alejandro; Lérida Viso, A.; Avellanal Zaballa, Edurne; Sola Llano, rebeca; Bañuelos, Jorge; Rodríguez Agarrabeitia, Antonia; Martínez Mañez, Ramon; Ortiz García, María JosefaWe have designed and synthesized a family of Ir(III) metal complexes coordinated with two cyclometalated bisfluorophenylpyridine ligands and an ancillary dipyrromethene which is functionalized with a mesityl group (Ir(dipy)-1), an α-chloroacetyl ester (Ir(dipy)-2) or a chain containing an ammonium cation (Ir(dipy)-3). The Ir (III) complexes feature a high triplet state population enabling red phosphorescence and efficient singlet oxygen generation. Ir(dipy)-2 and Ir(dipy)-3 are demonstrated to stain cells in both one-photon and two-photon confocal imaging. Moreover, Ir(dipy)-2 and Ir(dipy)-3 produce ROS in cells upon irradiation, inducing cell death by apoptosis. Colocalization studies in SK-Mel-103 cells show that Ir(dipy)-3 is partially accumulated in mitochondria and induces upon irradiation a disruption in their morphology. Overall our studies demonstrate that the prepared Ir(III) act as photosensitizers able to kill cells under irradiation, being suitable candidates for photodynamic therapy applications.Item BODIPY doping of covalent organic frameworks-based nanomaterials: A novel strategy towards biomedical applications(Dyes and Pigments, 2023) Suárez Blas, Fátima; Martínez Fernández, Marcos; Prieto Castañeda, Alejandro; García Fernández, Alba; Martínez Ruíz, José I.; Ramos Gallego, María Mar; Ortiz García, María Josefa; Martínez Máñez, Ramón; Segura Castedo, José LuisCovalent organic Frameworks (COFs) are a class of crystalline macromolecular materials build-up by monomers with specific symmetries or functionalities. There are important limitations in the synthesis of highly ordered COFs, such as the shape and packing of the building blocks. Thus, the presence of fluorine atoms that lie perpendicular to the bisecting plane of BODIPY derivatives together with the presence of four bulky methyl groups could hinder the crystallization process in COF synthesis. For that reason, BODIPY-based COFs are rarely incorporated to COF networks. In this work, following the mixed linker strategy, a pre-synthetic method to dope COF structures with BODIPY units was developed. The materials have been processed into fluorescent Covalent Organic Nanosheets (CONs) with defined particle-size distributions around 100 nm, suitable for cellular biomedical applications. The viability of the CONs was evaluated using Sk-Mel-103 cells, demonstrating the internalization showing 100% cell viability. We envisage that this work could accelerate the discovery of new COF-based materials for biomedical sciences