Person:
Domínguez Rodríguez, Lucas José

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First Name
Lucas José
Last Name
Domínguez Rodríguez
URI
https://hdl.handle.net/20.500.14352/80552
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Sanidad Animal
Area
Sanidad Animal
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UCM identifierORCIDScopus Author IDWeb of Science ResearcherIDDialnet IDGoogle Scholar ID

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2007 - 200922010 - 20181
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    Rapid differentiation of Staphylococcus aureus subspecies based on MALDI-TOF MS profiles
    (2018) Pérez Sancho, Marta; Vela Alonso, Ana Isabel; Horcajo Iglesias, María Del Pilar; Ugarte Ruiz, María; Domínguez Rodríguez, Lucas José; Fernández-Garayzábal Fernández, José Francisco; Fuente López, Ricardo De La
    Staphylococcus aureus encompasses 2 subspecies ( aureus and anaerobius) with significant differences in their epidemiology and pathogenicity. We evaluated the suitability of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the rapid identification of both subspecies using a panel of 52 S. aureus isolates (30 subsp. anaerobius and 22 subsp. aureus) recovered from different origins, countries, and years. The on-board library identification system correctly identified 42 of 52 (81%) S. aureus isolates at the species level with score values >2.0. Limited performance was observed for differentiation of S. aureus subspecies (particularly subsp. anaerobius). Visual inspection of MALDI-TOF MS profiles identified 5 subspecies-specific mass peaks ( m/ z 3430 and 6861 in S. aureus subsp. anaerobius, and m/ z 4046, 6890, and 8093 in S. aureus subsp. aureus) with 100% sensitivity and specificity values, which is potentially useful for differentiating these subspecies. The suitability of 3 models, Genetic Analysis (GA), Quick Classifier (QC), and Supervised Neural Network, for automatic identification of both subspecies was evaluated using the Recognition Capability (RC) and Cross Validation (CV) values provided by the on-board ClinProTools software. The GA and QC models reached RC and CV values of 100%. Both models were externally validated using a panel of 26 S. aureus isolates of both subspecies, with both models correctly classifying all isolates of both subspecies. MALDI-TOF MS coupled with ClinProTools software represents a rapid and simple approach for S. aureus subspecies discrimination.
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    β-Lactam resistance in Haemophilus parasuis is mediated by plasmid pB1000 bearing blaROB-1
    (Antimicrobial Agents and Chemotherapy, 2007) San Millán, Álvaro; Catalán, Ana; Nieto, Silvia; Farelo, Fidel; Gibert, Magdalena; Escudero García-Calderón, José Antonio; Moreno Romo, Miguel Ángel; Domínguez Rodríguez, Lucas José; González Zorn, Bruno
    beta-Lactam resistance in Haemophilus parasuis is an emerging phenomenon that has not yet been characterized from a molecular perspective. Clinical high-level beta-lactam-resistant isolates from Spain bore a novel plasmid, pB1000, expressing a functionally active ROB-1 beta-lactamase. Pulsed-field gel electrophoresis was applied for the first time to H. parasuis and showed that beta-lactam resistance is due to clonal spread of a resistant strain, BB1018, bearing pB1000.
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    First characterization of fluoroquinolone resistance in Streptococcus suis
    (Antimicrobial Agents and Chemotherapy, 2007) San Millán, Álvaro; Catalán, Ana; González de la Campa, Adela; Rivero, Estefanía; Lopez, Gema; Escudero García-Calderón, José Antonio; Domínguez Rodríguez, Lucas José; Moreno Romo, Miguel Ángel; González Zorn, Bruno
    We have identified and sequenced the genes encoding the quinolone-resistance determining region (QRDR) of ParC and GyrA in fluoroquinolone-susceptible and -resistant Streptococcus suis clinical isolates. Resistance is the consequence of single point mutations in the QRDRs of ParC and GyrA and is not due to clonal spread of resistant strains or horizontal gene transfer with other bacteria.