Brown marine algae Gongolaria baccata extract protects Caco-2 cells from oxidative stress induced by tert-butyl hydroperoxide

Martínez Caballero, María Aranzazu; Ares Lombán, Irma; Martínez Caballero, Marta; López Torres, Bernardo; Maximiliano Guerra, Jorge Enrique; Rodriguez, J.L; Martínez Larrañaga, María Rosa; Anadón Navarro, Arturo Ramón; Peteiro, C.; Rubiño, S; Hortos, M

Brown marine algae Gongolaria baccata extract protects Caco-2 cells from oxidative stress induced by tert-butyl hydroperoxide

Download

Brown marine algae Gongolaria baccata extract protects Caco-2 cells from.pdf (716.33 KB)

Official URL

https://www.sciencedirect.com/science/article/pii/S0278691521004932

Publication date

2021

Authors

Martínez Caballero, María Aranzazu

Ares Lombán, Irma

Martínez Caballero, Marta

López Torres, Bernardo

Maximiliano Guerra, Jorge Enrique

Rodriguez, J.L

Martínez Larrañaga, María Rosa

Anadón Navarro, Arturo Ramón

Peteiro, C.

Rubiño, S

Publisher

Elsevier

Citations

Exportar

URI

https://hdl.handle.net/20.500.14352/97677

Citation

Martínez MA, Ares I, Martínez M, Lopez-Torres B, Maximiliano JE, Rodríguez JL, Martínez-Larrañaga MR, Anadón A, Peteiro C, Rubiño S, Hortos M. Brown marine algae Gongolaria baccata extract protects Caco-2 cells from oxidative stress induced by tert-butyl hydroperoxide. Food Chem Toxicol. 2021 Oct;156:112460. doi: 10.1016/j.fct.2021.112460. Epub 2021 Aug 1. PMID: 34348126.

Abstract

Gongolaria baccata (S.G. Gmelin) is marine brown seaweed mainly found on the coasts of the Baltic Sea south to the Mediterranean Sea, Canary Islands, Mauritania and Western Sahara. Herein, we report the cell viability and protective effects attributed to molecular mechanisms underlying antioxidant response to survive oxidative stress injuries. Caco-2 cells were submitted to oxidative stress by treatment with tert-butylhydroperoxide (tert-BOOH). The extract prevented cell damage and enhanced activity of antioxidant defenses (NQO1 and GST activities and GSH levels) reduced by treatment with tert-BOOH. The increases of MDA levels, the amount of intracellular ROS and caspase 3/7 activity induced by tert-BOOH were prevented when cells were treated with the G. baccata extract. Moreover, G. baccata extract caused up-regulation of GSTM2, Nrf2, and AKT1 gene expressions, as well as G. baccata extract reduced significantly Bax, BNIP3, APAF1, ERK1, JNK1, MAPK1, P38, P53, NFκB1, TNFα, IL-6, IL-1β and HO-1 gene expressions related to apoptosis, proinflammation and oxidative stress induced by tert-BOOH. These results suggest that G.baccata extract protected the cells against oxidative damage and inflammation; protective effects that could be linked to their bioactive constituents. Hence, this brown seaweed G. baccata extract could be used for the development of functional foods and/or nutraceuticals.