Fast label-free optical diffraction tomography compatible with conventional wide-field microscopes

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dc.book.titleOptical Methods for Inspection, Characterization, and Imaging of Biomaterials IV
dc.contributor.authorRodrigo Martín-Romo, José Augusto
dc.contributor.authorSoto Rueda, Juan Manuel
dc.contributor.authorAlieva Krasheninnikova, Tatiana
dc.date.accessioned2023-06-17T14:18:20Z
dc.date.available2023-06-17T14:18:20Z
dc.date.issued2019
dc.description© 2019 Society of Photo-Optical Instrumentation Engineers (SPIE). Optical Methods for Inspection, Characterization, and Imaging of Biomaterials (4º. 2019. Munich) ISSN 0277-786X. The funding of the projects TEC2014-57394-P (Ministerio de Economía y Competitividad) and PR75/18-21567 (Santander-UCM) is acknowledged.
dc.description.abstractPartially coherent optical diffraction tomography (PC-ODT) is a labelfree quantitative 3D imaging technique based on the refractive index contrast. It provides fast non-interferometric speckle noise-free imaging compatible with conventional wide-field transmission microscopes, but suffers from two principal inconveniences. One of them is the missing cone problem, which is a common drawback for all tomographic modalities with limitedangle inspection, including interferometric coherent ODT. The second one, considered in this paper, is a non-homogeneous contrast for different spatial frequency regions. Analyzing the microscope 3D optical transfer functions for various illuminations over the condenser aperture we have found that the Gaussian illumination shape is a proper one. Numerical simulations and experimental results support this finding. A future line in the development of post-deconvolution processing is also discussed.
dc.description.departmentDepto. de Óptica
dc.description.facultyFac. de Ciencias Físicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y Competitividad (MINECO)
dc.description.sponsorshipUniversidad Complutense de Madrid/Banco de Santander
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/58614
dc.identifier.doi10.1117/12.2525840
dc.identifier.isbn978-1-5106-2800-7
dc.identifier.issn0277-786X
dc.identifier.officialurlhttp://dx.doi.org/10.1117/12.2525840
dc.identifier.relatedurlhttps://www.spiedigitallibrary.org
dc.identifier.urihttps://hdl.handle.net/20.500.14352/14029
dc.issue.number11060
dc.language.isoeng
dc.page.total11
dc.publication.placeBelliningham, Washington
dc.publisherSociety of Photo-Optical Instrumentation Engineers (SPIE)
dc.relation.ispartofseriesProceedings of SPIE
dc.relation.projectIDTEC2014-57394-P
dc.relation.projectIDPR75/18-21567
dc.rights.accessRightsopen access
dc.subject.cdu535
dc.subject.keywordPartially coherent-Light
dc.subject.keywordReconstruction
dc.subject.keywordAlgorithms
dc.subject.ucmÓptica (Física)
dc.subject.unesco2209.19 Óptica Física
dc.titleFast label-free optical diffraction tomography compatible with conventional wide-field microscopes
dc.typebook part
dspace.entity.typePublication
relation.isAuthorOfPublication744b2ca4-d506-4370-b237-ee577fd591a0
relation.isAuthorOfPublicationf1512137-328a-4bb6-9714-45de778c1be4
relation.isAuthorOfPublication.latestForDiscovery744b2ca4-d506-4370-b237-ee577fd591a0
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