Fast label-free optical diffraction tomography compatible with conventional wide-field microscopes
dc.book.title | Optical Methods for Inspection, Characterization, and Imaging of Biomaterials IV | |
dc.contributor.author | Rodrigo Martín-Romo, José Augusto | |
dc.contributor.author | Soto Rueda, Juan Manuel | |
dc.contributor.author | Alieva Krasheninnikova, Tatiana | |
dc.date.accessioned | 2023-06-17T14:18:20Z | |
dc.date.available | 2023-06-17T14:18:20Z | |
dc.date.issued | 2019 | |
dc.description | © 2019 Society of Photo-Optical Instrumentation Engineers (SPIE). Optical Methods for Inspection, Characterization, and Imaging of Biomaterials (4º. 2019. Munich) ISSN 0277-786X. The funding of the projects TEC2014-57394-P (Ministerio de Economía y Competitividad) and PR75/18-21567 (Santander-UCM) is acknowledged. | |
dc.description.abstract | Partially coherent optical diffraction tomography (PC-ODT) is a labelfree quantitative 3D imaging technique based on the refractive index contrast. It provides fast non-interferometric speckle noise-free imaging compatible with conventional wide-field transmission microscopes, but suffers from two principal inconveniences. One of them is the missing cone problem, which is a common drawback for all tomographic modalities with limitedangle inspection, including interferometric coherent ODT. The second one, considered in this paper, is a non-homogeneous contrast for different spatial frequency regions. Analyzing the microscope 3D optical transfer functions for various illuminations over the condenser aperture we have found that the Gaussian illumination shape is a proper one. Numerical simulations and experimental results support this finding. A future line in the development of post-deconvolution processing is also discussed. | |
dc.description.department | Depto. de Óptica | |
dc.description.faculty | Fac. de Ciencias Físicas | |
dc.description.refereed | TRUE | |
dc.description.sponsorship | Ministerio de Economía y Competitividad (MINECO) | |
dc.description.sponsorship | Universidad Complutense de Madrid/Banco de Santander | |
dc.description.status | pub | |
dc.eprint.id | https://eprints.ucm.es/id/eprint/58614 | |
dc.identifier.doi | 10.1117/12.2525840 | |
dc.identifier.isbn | 978-1-5106-2800-7 | |
dc.identifier.issn | 0277-786X | |
dc.identifier.officialurl | http://dx.doi.org/10.1117/12.2525840 | |
dc.identifier.relatedurl | https://www.spiedigitallibrary.org | |
dc.identifier.uri | https://hdl.handle.net/20.500.14352/14029 | |
dc.issue.number | 11060 | |
dc.language.iso | eng | |
dc.page.total | 11 | |
dc.publication.place | Belliningham, Washington | |
dc.publisher | Society of Photo-Optical Instrumentation Engineers (SPIE) | |
dc.relation.ispartofseries | Proceedings of SPIE | |
dc.relation.projectID | TEC2014-57394-P | |
dc.relation.projectID | PR75/18-21567 | |
dc.rights.accessRights | open access | |
dc.subject.cdu | 535 | |
dc.subject.keyword | Partially coherent-Light | |
dc.subject.keyword | Reconstruction | |
dc.subject.keyword | Algorithms | |
dc.subject.ucm | Óptica (Física) | |
dc.subject.unesco | 2209.19 Óptica Física | |
dc.title | Fast label-free optical diffraction tomography compatible with conventional wide-field microscopes | |
dc.type | book part | |
dspace.entity.type | Publication | |
relation.isAuthorOfPublication | 744b2ca4-d506-4370-b237-ee577fd591a0 | |
relation.isAuthorOfPublication | f1512137-328a-4bb6-9714-45de778c1be4 | |
relation.isAuthorOfPublication.latestForDiscovery | 744b2ca4-d506-4370-b237-ee577fd591a0 |
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