Fast label-free optical diffraction tomography compatible with conventional wide-field microscopes
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2019
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Society of Photo-Optical Instrumentation Engineers (SPIE)
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Abstract
Partially coherent optical diffraction tomography (PC-ODT) is a labelfree quantitative 3D imaging technique based on the refractive index contrast. It provides fast non-interferometric speckle noise-free imaging compatible with conventional wide-field transmission microscopes, but suffers from two principal inconveniences. One of them is the missing cone problem, which is a common drawback for all tomographic modalities with limitedangle inspection, including interferometric coherent ODT. The second one, considered in this paper, is a non-homogeneous contrast for different spatial frequency regions. Analyzing the microscope 3D optical transfer functions for various illuminations over the condenser aperture we have found that the Gaussian illumination shape is a proper one. Numerical simulations and experimental results support this finding. A future line in the development of post-deconvolution processing is also discussed.
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© 2019 Society of Photo-Optical Instrumentation Engineers (SPIE).
Optical Methods for Inspection, Characterization, and Imaging of Biomaterials (4º. 2019. Munich)
ISSN 0277-786X.
The funding of the projects TEC2014-57394-P (Ministerio de Economía y Competitividad) and PR75/18-21567 (Santander-UCM) is acknowledged.