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Identification of waterborne bacteria by the analysis of 16S–23S rRNA intergenic spacer region

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dc.contributor.authorPérez Luz, Sara
dc.contributor.authorYañez, María Adela
dc.contributor.authorCatalan, Vicente
dc.date.accessioned2024-01-22T15:02:22Z
dc.date.available2024-01-22T15:02:22Z
dc.date.issued2004
dc.description.abstractAim: In this study, we evaluated, the use of universal primers, specific for the 16S-23S rRNA intergenic region, to detect and identify nine species that are of high interest for the microbiological control of water. Methods and results: The analysis of the fragments was carried out using a High Resolution acrylamide/bisacrylamide gels in a fluorescent automated DNA sequencer. The results showed specific profiles for each of the nine species but this technique failed to detect simultaneously micro-organisms in samples containing a mixed population. Conclusion: Nevertheless, the electrophoretic profiles obtained provided a very useful tool for the rapid and specific identification of water isolates. Significance and impact of the study: A possible new methodology for a rapid identification of pathogens in water.
dc.description.departmentDepto. de Bioquímica y Biología Molecular
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia y Tecnología (España)
dc.description.sponsorshipMinisterio de Industria y Energía (España)
dc.description.statuspub
dc.identifier.citationPérez-Luz, S., Adela Yáñez, M., & Catalán, V. (2004). Identification of waterborne bacteria by the analysis of 16S-23S rRNA intergenic spacer region. Journal of applied microbiology, 97(1), 191–204. https://doi.org/10.1111/j.1365-2672.2004.02288.x
dc.identifier.doi10.1111/j.1365-2672.2004.02288.x
dc.identifier.essn1365-2672
dc.identifier.officialurlhttps://doi.org/10.1111/j.1365-2672.2004.02288.x
dc.identifier.urihttps://hdl.handle.net/20.500.14352/94437
dc.issue.number1
dc.journal.titleJournal of Applied Microbiology
dc.language.isoeng
dc.page.final204
dc.page.initial191
dc.publisherWiley
dc.relation.projectIDPM 1999/0078
dc.relation.projectIDB185/1·999
dc.relation.projectIDB221/1·998
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsrestricted access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.cdu57
dc.subject.keyword16S-23S
dc.subject.keywordBacteria
dc.subject.keywordIdentification
dc.subject.keywordITS
dc.subject.keywordrRNA
dc.subject.keywordWaterborne
dc.subject.ucmBiología
dc.subject.unesco2415 Biología Molecular
dc.titleIdentification of waterborne bacteria by the analysis of 16S–23S rRNA intergenic spacer region
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number97
dspace.entity.typePublication
relation.isAuthorOfPublication45168e0c-c225-4385-9386-0e750ca8ee09
relation.isAuthorOfPublication.latestForDiscovery45168e0c-c225-4385-9386-0e750ca8ee09

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