Identification of waterborne bacteria by the analysis of 16S–23S rRNA intergenic spacer region
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2004
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Wiley
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Pérez-Luz, S., Adela Yáñez, M., & Catalán, V. (2004). Identification of waterborne bacteria by the analysis of 16S-23S rRNA intergenic spacer region. Journal of applied microbiology, 97(1), 191–204. https://doi.org/10.1111/j.1365-2672.2004.02288.x
Abstract
Aim: In this study, we evaluated, the use of universal primers, specific for the 16S-23S rRNA intergenic region, to detect and identify nine species that are of high interest for the microbiological control of water.
Methods and results: The analysis of the fragments was carried out using a High Resolution acrylamide/bisacrylamide gels in a fluorescent automated DNA sequencer. The results showed specific profiles for each of the nine species but this technique failed to detect simultaneously micro-organisms in samples containing a mixed population.
Conclusion: Nevertheless, the electrophoretic profiles obtained provided a very useful tool for the rapid and specific identification of water isolates.
Significance and impact of the study: A possible new methodology for a rapid identification of pathogens in water.