Publication:
Proteomic Characterization of Bacteriophage Peptides from the Mastitis Producer Staphylococcus aureus by LC-ESI-MS/MS and the Bacteriophage Phylogenomic Analysis

dc.contributor.authorAbril, Ana G.
dc.contributor.authorCarrera, Mónica
dc.contributor.authorBöhme, Karola
dc.contributor.authorBarros-Velázquez, Jorge
dc.contributor.authorCañas, Benito
dc.contributor.authorRama, Jose Luis R.
dc.contributor.authorVilla, Tomás G.
dc.contributor.authorCalo-Mata, Pilar
dc.date.accessioned2023-06-17T08:34:50Z
dc.date.available2023-06-17T08:34:50Z
dc.date.issued2021
dc.description.abstractThe present work describes LC-ESI-MS/MS MS (liquid chromatography-electrospray ionization-tandem mass spectrometry) analyses of tryptic digestion peptides from phages that infect mastitis-causing Staphylococcus aureus isolated from dairy products. A total of 1933 nonredundant peptides belonging to 1282 proteins were identified and analyzed. Among them, 79 staphylococcal peptides from phages were confirmed. These peptides belong to proteins such as phage repressors, structural phage proteins, uncharacterized phage proteins and complement inhibitors. Moreover, eighteen of the phage origin peptides found were specific to S. aureus strains. These diagnostic peptides could be useful for the identification and characterization of S. aureus strains that cause mastitis. Furthermore, a study of bacteriophage phylogeny and the relationship among the identified phage peptides and the bacteria they infect was also performed. The results show the specific peptides that are present in closely related phages and the existing links between bacteriophage phylogeny and the respective Staphylococcus spp. infected.
dc.description.departmentDepto. de Química Analítica
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y Competitividad
dc.description.sponsorshipGAIN-Xunta de Galicia
dc.description.sponsorshipSpanish AEI/EU-FEDER
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/78611
dc.identifier.doi10.3390/foods10040799
dc.identifier.issn2304-8158
dc.identifier.officialurlhttps://doi.org/10.3390/foods10040799
dc.identifier.urihttps://hdl.handle.net/20.500.14352/7483
dc.journal.titleFoods
dc.language.isoeng
dc.publisherMDPI
dc.relation.projectIDProject AGL 2.013-48.244-R
dc.relation.projectIDProject (IN607D 2017/01)
dc.relation.projectIDPID2019-103845RB-C21
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/es/
dc.subject.cdu543
dc.subject.keywordpathogen detection
dc.subject.keywordLC-ESI-MS/MS
dc.subject.keywordproteomics
dc.subject.keywordmass spectrometry
dc.subject.keywordphage peptide biomarker
dc.subject.ucmQuímica analítica (Química)
dc.subject.unesco2301 Química Analítica
dc.titleProteomic Characterization of Bacteriophage Peptides from the Mastitis Producer Staphylococcus aureus by LC-ESI-MS/MS and the Bacteriophage Phylogenomic Analysis
dc.typejournal article
dc.volume.number10
dspace.entity.typePublication
Files
Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
foods-10-00799.pdf
Size:
5.55 MB
Format:
Adobe Portable Document Format
Collections