An array-based electrochemical magneto-immunosensor for early neonatal sepsis diagnostic: Fast and accurate determination of C-reactive protein in whole blood and plasma samples

Molinero-Fernández, Águeda; Moreno Guzmán, María; López, Miguel Ángel; Escarpa, Alberto

An array-based electrochemical magneto-immunosensor for early neonatal sepsis diagnostic: Fast and accurate determination of C-reactive protein in whole blood and plasma samples

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Official URL

https://www.sciencedirect.com/science/article/pii/S0026265X20306937

Publication date

2020

Authors

Molinero-Fernández, Águeda

Moreno Guzmán, María

López, Miguel Ángel

Escarpa, Alberto

Publisher

Elsevier

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URI

https://hdl.handle.net/20.500.14352/93025

Abstract

An array-based electrochemical magneto-immunosensor (a-EMI) constituted by eight screen-printed carbon electrodes is proposed for the determination of C-reactive protein (CRP). Remarkably, the analytical usefulness of the approach was demonstrated not only by analyzing whole blood samples, but plasma ones from newborns with suspicion of sepsis, which sample volume is hardly available. The a-EIM enabled the accurate determination of CRP in these unique samples (Er < 3%) with excellent intra (CV<4%, n=8) and inter-assays (CV<9%, n=8) precision, using only 5 µL of clinical sample, covering the sepsis diagnostic range and employing only 15 min for total assay. The electrode array enabled to work with up to 8 tailored dilutions simultaneously accordingly to the CRP clinical diagnostic range. It constitutes by itself a valuable advantage for speed and reliability improvements in the sample analysis and further sepsis diagnosis. These results non-only highly met the clinical needs for sepsis newborns diagnosis, but they opened new perspectives in low volumes-based diagnostics.