New species genetic approach to identify strains of mitis group Streptococci that are donors of rifampin resistance to Streptococcus pneumoniae
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2011
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Ferrándiz, M.-J., Ardanuy, C., Liñares, J., Balsalobre, L., García, M. T., & De La Campa, A. G. (2011). New species genetic approach to identify strains of mitis group streptococci that are donors of rifampin resistance to Streptococcus pneumoniae. Antimicrobial Agents and Chemotherapy, 55(1), 368-372. https://doi.org/10.1128/AAC.00856-10
Abstract
Eight rifampin-resistant streptococci of the mitis group were identified at the species level by using a concatenated 16S rRNA gene-sodA-rpoB-hlpA sequence. Characterization of their rpoB alleles showed single amino acid changes involved in rifampin resistance. Comparison of RpoB sequences from pneumococcal recombinant isolates, viridans isolates, and type strains revealed a species-specific amino acid signature, which allowed it to be ascertained that recombinant RpoBs were originated in genetic interchanges with Streptococcus mitis and Streptococcus oralis.
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This work was supported by Plan Nacional de IDI of Ministerio de Ciencia e Innovación (grant no. BIO2008-02154) and Comunidad de Madrid (grant no. COMBACT-S-BIO-0260/2006).