Development of an Optimal Protocol for the Proteomic Analysis of Stenotic and Healthy Aortic Valves

Gil Dones, Félix; Martín-Rojas, Tatiana; López-Almodovar, Luis; Juárez-Tosina, Rocío; Cuesta, Fernando de La; Álvarez Llamas, Gloria; Alonso-Orgaz, Sergio; Vivanco, Fernando; Rodríguez-Padial, Luis; Barderas, María

Development of an Optimal Protocol for the Proteomic Analysis of Stenotic and Healthy Aortic Valves

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Official URL

https://doi.org/10.1016/s1885-5857(10)70008-6

Publication date

2010

Publisher

Elsevier

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URI

https://hdl.handle.net/20.500.14352/94681

Citation

Gil-Dones, Félix, et al. «Development of an Optimal Protocol for the Proteomic Analysis of Stenotic and Healthy Aortic Valves». Revista Española de Cardiología (English Edition), vol. 63, n.o 1, enero de 2010, pp. 46-53. https://doi.org/10.1016/S1885-5857(10)70008-6.

Abstract

Introduction and objectives: For many years, degenerative aortic stenosis was thought to be a passive process secondary to calcium deposition in aortic valves. Although its etiology remains unknown, several authors have pointed out that degenerative aortic stenosis is associated with the same risk factors as coronary artery disease. Furthermore, histological similarities have been found between aortic valve stenosis and atherosclerotic plaque, giving rise to the hypothesis that degenerative aortic stenosis is an inflammatory process similar to atherosclerosis. Nevertheless, some data do not fit with this hypothesis and, consequently, greater understanding of the condition is needed. The main aim of this study was to develop a practical protocol for extracting protein for use in proteomic analysis from both stenotic and healthy aortic valves. Methods: The study was carried out using a number of different proteomic methods: two-dimensional electrophoresis, mass spectrometry, and additional techniques. Results: We developed a simple and reproducible methodology in the laboratory for carrying out the proteomic analysis of human aortic valves and for identifying their component proteins. Conclusions: We developed a simple and reproducible method for extracting protein that can be used with mass spectrometry and that makes it possible to carry out large-scale proteomic analysis of stenotic aortic valves. Furthermore, the methodology will significantly increase our understanding of the valve proteome.

Description

This study was conducted with the financial help of the Esteve Grant awarded by the Spanish Society of Cardiology. Redes Técnicas de Investigación Cooperativa (RETICS), Instituto de Salud Carlos III, RD06/0014/1015. Health Research Fund PI070537 and P080970; CAM (Biomarkers S2006/GENE-0247). FISCAM 2008/08.