Development of an Optimal Protocol for the Proteomic Analysis of Stenotic and Healthy Aortic Valves

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dc.contributor.authorGil Dones, Félix
dc.contributor.authorMartín-Rojas, Tatiana
dc.contributor.authorLópez-Almodovar, Luis
dc.contributor.authorJuárez-Tosina, Rocío
dc.contributor.authorCuesta, Fernando de La
dc.contributor.authorÁlvarez Llamas, Gloria
dc.contributor.authorAlonso-Orgaz, Sergio
dc.contributor.authorVivanco, Fernando
dc.contributor.authorRodríguez-Padial, Luis
dc.contributor.authorBarderas, María
dc.date.accessioned2024-01-23T11:06:12Z
dc.date.available2024-01-23T11:06:12Z
dc.date.issued2010
dc.descriptionThis study was conducted with the financial help of the Esteve Grant awarded by the Spanish Society of Cardiology. Redes Técnicas de Investigación Cooperativa (RETICS), Instituto de Salud Carlos III, RD06/0014/1015. Health Research Fund PI070537 and P080970; CAM (Biomarkers S2006/GENE-0247). FISCAM 2008/08.
dc.description.abstractIntroduction and objectives: For many years, degenerative aortic stenosis was thought to be a passive process secondary to calcium deposition in aortic valves. Although its etiology remains unknown, several authors have pointed out that degenerative aortic stenosis is associated with the same risk factors as coronary artery disease. Furthermore, histological similarities have been found between aortic valve stenosis and atherosclerotic plaque, giving rise to the hypothesis that degenerative aortic stenosis is an inflammatory process similar to atherosclerosis. Nevertheless, some data do not fit with this hypothesis and, consequently, greater understanding of the condition is needed. The main aim of this study was to develop a practical protocol for extracting protein for use in proteomic analysis from both stenotic and healthy aortic valves. Methods: The study was carried out using a number of different proteomic methods: two-dimensional electrophoresis, mass spectrometry, and additional techniques. Results: We developed a simple and reproducible methodology in the laboratory for carrying out the proteomic analysis of human aortic valves and for identifying their component proteins. Conclusions: We developed a simple and reproducible method for extracting protein that can be used with mass spectrometry and that makes it possible to carry out large-scale proteomic analysis of stenotic aortic valves. Furthermore, the methodology will significantly increase our understanding of the valve proteome.
dc.description.departmentDepto. de Genética, Fisiología y Microbiología
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.sponsorshipSpanish Society of Cardiology
dc.description.sponsorshipComunidad de Madrid
dc.description.sponsorshipInstituto de Salud Carlos III
dc.description.statuspub
dc.identifier.citationGil-Dones, Félix, et al. «Development of an Optimal Protocol for the Proteomic Analysis of Stenotic and Healthy Aortic Valves». Revista Española de Cardiología (English Edition), vol. 63, n.o 1, enero de 2010, pp. 46-53. https://doi.org/10.1016/S1885-5857(10)70008-6.
dc.identifier.doi10.1016/s1885-5857(10)70008-6
dc.identifier.essn1579-2242
dc.identifier.issn0300-8932
dc.identifier.officialurlhttps://doi.org/10.1016/s1885-5857(10)70008-6
dc.identifier.urihttps://hdl.handle.net/20.500.14352/94681
dc.issue.number1
dc.journal.titleRevista Española de Cardiologia
dc.language.isospa
dc.page.final53
dc.page.initial46
dc.publisherElsevier
dc.rights.accessRightsrestricted access
dc.subject.cdu577.112
dc.subject.cdu577.21
dc.subject.cdu616.12
dc.subject.keywordProteomics
dc.subject.keywordAortic stenosis
dc.subject.keywordBiomarkers
dc.subject.ucmBioquímica (Biología)
dc.subject.ucmGenética
dc.subject.ucmCardiología
dc.subject.unesco2403 Bioquímica
dc.subject.unesco2409 Genética
dc.subject.unesco3213 Cirugía
dc.titleDevelopment of an Optimal Protocol for the Proteomic Analysis of Stenotic and Healthy Aortic Valves
dc.title.alternativeObtención de un protocolo óptimo para el análisis proteómico de válvulas aórticas humanas sanas y estenóticas
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number63
dspace.entity.typePublication
relation.isAuthorOfPublication0827e638-921a-4475-9a48-b859587719c5
relation.isAuthorOfPublication16e9b128-f6de-4009-817c-53e91375baa3
relation.isAuthorOfPublication.latestForDiscovery0827e638-921a-4475-9a48-b859587719c5
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