Paving the Way for Reliable Alzheimer’s Disease Blood Diagnosis by Quadruple Electrochemical Immunosensing

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dc.contributor.authorValverde de la Fuente, Alejandro
dc.contributor.authorGordón Pidal, José María
dc.contributor.authorMontero Calle, Ana
dc.contributor.authorArévalo Pérez, Beatriz
dc.contributor.authorSerafín González-Carrato, Verónica
dc.contributor.authorCalero, Miguel
dc.contributor.authorMoreno Guzmán, María
dc.contributor.authorLópez, Miguel Ángel
dc.contributor.authorEscarpa, Alberto
dc.contributor.authorYáñez Sedeño, Paloma
dc.contributor.authorBarderas, Rodrigo
dc.contributor.authorCampuzano Ruiz, Susana
dc.contributor.authorPingarrón Carrazón, José Manuel
dc.date.accessioned2023-06-22T10:41:15Z
dc.date.available2023-06-22T10:41:15Z
dc.date.issued2022-02-01
dc.descriptionCRUE-CSIC (Acuerdos Transformativos 2022)
dc.description.abstractAlzheimer’s disease (AD), the most common neurodegenerative disorder, demands new cost-effective and easy-to-use strategies for its reliable detection, mainly in the preclinical stages. Here, we report the first immunoplatform for the electrochemical multidetermination of four candidate protein biomarkers in blood, neurofilament light chain (NfL), Tau, phosphorylated Tau (p-Tau) and TAR DNA-Binding Protein 43 (TDP-43). It involves implementation of sandwich-type immunoassays and enzymatic labelling with horseradish peroxidase (HRP) on the surface of magnetic microbeads (MBs). Amperometric detection is performed after depositing the magnetic immunoconjugates on disposable quadruple transduction platforms by monitoring the enzymatic reduction of H2O2 mediated by hydroquinone (HQ). The immunoplatform achieved LOD values smaller than the content of target biomarkers in plasma of healthy subjects, with RSD values.
dc.description.departmentDepto. de Química Analítica
dc.description.departmentDepto. de Química en Ciencias Farmacéuticas
dc.description.facultyFac. de Ciencias Químicas
dc.description.facultyFac. de Farmacia
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia e Innovación (MICINN)
dc.description.sponsorshipInstituto de Salud Carlos III (ISCIII)
dc.description.sponsorshipComunidad de Madrid
dc.description.sponsorshipI
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/71456
dc.identifier.doi10.1002/celc.202200055
dc.identifier.issn2196-0216
dc.identifier.officialurlhttps://doi.org/10.1002/celc.202200055
dc.identifier.urihttps://hdl.handle.net/20.500.14352/71373
dc.journal.titleChemElectroChem
dc.language.isoeng
dc.publisherWiley
dc.relation.projectIDPID2019-103899RB-I00; RTI2018-096135-B-I00; PID2020- 118154GB-I00; (PRE2019- 087596)
dc.relation.projectIDPI17CIII/00045; PI20CIII/00019
dc.relation.projectIDTRANSNANOAVANSENS-CM (PS2018/NMT-4349)
dc.rightsAtribución-NoComercial 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by-nc/3.0/es/
dc.subject.keywordAlzheimer’s disease
dc.subject.keywordCandidate biomarkers
dc.subject.keywordElectrochemical
dc.subject.keywordmultiplexing immunoplatform
dc.subject.keywordMagnetic microbeads
dc.subject.keywordPlasma
dc.subject.ucmQuímica
dc.subject.ucmQuímica analítica (Química)
dc.subject.ucmNeurociencias (Medicina)
dc.subject.unesco23 Química
dc.subject.unesco2301 Química Analítica
dc.subject.unesco2490 Neurociencias
dc.titlePaving the Way for Reliable Alzheimer’s Disease Blood Diagnosis by Quadruple Electrochemical Immunosensing
dc.typejournal article
dspace.entity.typePublication
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relation.isAuthorOfPublication.latestForDiscoveryd96495de-d573-44a0-9b16-15cacb73baf4
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