Early in vitro response of macrophages and T lymphocytes to nanocrystalline hydroxyapatites

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dc.contributor.authorMatesanz Sancho, María Concepción
dc.contributor.authorFeito Castellano, María José
dc.contributor.authorOñaderra Sánchez, Mercedes
dc.contributor.authorRamirez Santillán, Cecilia
dc.contributor.authorCasa, Carmen da
dc.contributor.authorArcos Navarrete, Daniel
dc.contributor.authorRojo, José María
dc.contributor.authorVallet Regí, María Dulce Nombre
dc.contributor.authorPortolés Pérez, María Teresa
dc.date.accessioned2023-06-19T15:06:50Z
dc.date.available2023-06-19T15:06:50Z
dc.date.issued2014-02-15
dc.descriptionRESEARCHER ID M-3378-2014 (María Vallet Regí) ORCID 0000-0002-6104-4889 (María Vallet Regí)
dc.description.abstractHypothesis: Synthetic hydroxyapatite (HA) and Si substituted hydroxyapatite (SiHA) are calcium phosphate ceramics currently used in the field of dentistry and orthopaedic surgery. The preparation of both biomaterials as polycrystalline solid pieces or grains formed by nanocrystallites has awakened a great interest to enhance the bioactive behavior due to the microstructural defects and the higher surface area. The study of the macrophage and lymphocyte behavior in contact with nanocrystalline HA and SiHA will allow to elucidate the immune response which conditions the success or rejection of these biomaterials. Experiments: HA and SiHA granules (with sizes of tens of microns) have been prepared by controlled aqueous precipitation avoiding subsequent high temperature sintering. HA and SiHA granules were constituted by crystallites smaller than 50 nm. The effects of both nanocrystalline materials on immune system have been evaluated with macrophages (main components of innate immune system) and T lymphocytes (specific cells of adaptive response) after short-term culture as in vitro models of the early immune response. Findings: Significant decreases of macrophage proliferation and phagocytic activity, increased production of inflammatory cytokines (IL-6, TNF-a) and T lymphocyte apoptosis, were induced by these nanocrystalline ceramics suggesting that, after in vivo implantation, they induce significant effects on immune responses, including an early activation of the innate immune system. (C) 2013 Elsevier Inc. All rights reserved.
dc.description.departmentDepto. de Química en Ciencias Farmacéuticas
dc.description.facultyFac. de Farmacia
dc.description.refereedTRUE
dc.description.sponsorshipComunidad de Madrid
dc.description.sponsorshipMinisterio de Ciencia e Innovacion (MICINN)
dc.description.sponsorshipAgening Network of Excellence
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/40872
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dc.identifier.doi10.1016/j.jcis.2013.10.045
dc.identifier.issn0021-9797
dc.identifier.officialurlhttps://www.elsevier.com/
dc.identifier.urihttps://hdl.handle.net/20.500.14352/35362
dc.journal.titleJournal of Colloid and Interface Science
dc.language.isoeng
dc.page.final66
dc.page.initial59
dc.publisherElsevier
dc.relation.projectIDBITI (S2009/MAT-1472)
dc.relation.projectIDMAT2012-35556
dc.relation.projectIDCS02010-11384-E
dc.rights.accessRightsopen access
dc.subject.cdu546
dc.subject.cdu615.46
dc.subject.keywordBiocompatibility
dc.subject.keywordCytokine
dc.subject.keywordHydroxyapatite
dc.subject.keywordLymphocyte
dc.subject.keywordMacrophage
dc.subject.keywordSilicon-substituted hydroxyapatite
dc.subject.keywordMesoporous bioactive glass
dc.subject.keywordAntigen-presenting cells
dc.subject.keywordImmune-responses
dc.subject.keywordCalcium
dc.subject.keywordBiomaterials
dc.subject.keywordBioceramics
dc.subject.keywordOsteoblasts
dc.subject.keywordFibroblasts
dc.subject.ucmMateriales
dc.subject.ucmQuímica inorgánica (Química)
dc.subject.unesco3312 Tecnología de Materiales
dc.subject.unesco2303 Química Inorgánica
dc.titleEarly in vitro response of macrophages and T lymphocytes to nanocrystalline hydroxyapatites
dc.typejournal article
dc.volume.number416
dspace.entity.typePublication
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relation.isAuthorOfPublication.latestForDiscovery216318f7-e25a-4850-b122-856eb08b3e2f
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