Electrochemical immunosensor for receptor tyrosine kinase AXL using poly(pyrrolepropionic acid)-modified disposable electrodes

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dc.contributor.authorSerafín González-Carrato, Verónica
dc.contributor.authorTorrente Rodríguez, Rebeca Magnolia
dc.contributor.authorBatlle, Montserrat
dc.contributor.authorGarcía de Frutos, Pablo
dc.contributor.authorCampuzano Ruiz, Susana
dc.contributor.authorYáñez-Sedeño Orive, Paloma
dc.contributor.authorPingarrón Carrazón, José Manuel
dc.date.accessioned2024-01-25T10:56:24Z
dc.date.available2024-01-25T10:56:24Z
dc.date.issued2016
dc.description.abstractA sensitive and rapid method for the determination of the clinically relevant biomarker receptor tyrosine kinase AXL in serum involving amperometric disposable immunosensors is reported. The target protein was sandwiched between a specific capture antibody covalently immobilized on screen-printed carbon electrodes modified with electropolymerized poly(pyrrolepropionic acid) and a biotinylated detector antibody labeled with a streptavidin-horseradish peroxidase conjugate. The amperometric responses were measured at −0.20 V vs the Ag pseudo-reference electrode of the SPCE upon the addition of H2O2 in the presence of hydroquinone (HQ) as mediator. This integrated immunosensing platform showed a low limit of detection (337 pg mL−1), a good selectivity against other non-target serum proteins, and provided results statistically in agreement with those obtained by using a commercial ELISA kit. These attractive features together with the simplicity and easy automation and miniaturization of the required instrumentation make the developed methodology a promising alternative in the development of devices for on-site clinical analysis.
dc.description.departmentDepto. de Química Analítica
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Economía y Competitividad (España)
dc.description.sponsorshipEuropean Commission
dc.description.sponsorshipComunidad de Madrid
dc.description.statuspub
dc.identifier.citationSerafín V, Torrente-Rodríguez R.M., Batlle M, García de Frutos P, Campuzano S, Yáñez-Sedeño P, Pingarrón J.M. Electrochemical immunosensor for receptor tyrosine kinase AXL using poly(pyrrolepropionic acid)-modified disposable electrodes. Sens. Actuators, B. 2016 Septiembre 20; 240: 1251-56
dc.identifier.doi10.1016/j.snb.2016.09.109
dc.identifier.issn0925-4005
dc.identifier.officialurlhttps://doi.org/10.1016/j.snb.2016.09.109
dc.identifier.relatedurlhttps://produccioncientifica.ucm.es/documentos/5d9dafb5299952484ef81db7
dc.identifier.urihttps://hdl.handle.net/20.500.14352/95358
dc.journal.titleSensors and Actuators B: Chemical
dc.language.isoeng
dc.page.final1256
dc.page.initial1251
dc.publisherElsevier
dc.relation.projectIDRTC2015-4184-1
dc.relation.projectIDCTQ2015-70023-R
dc.relation.projectIDCTQ201564402-C2-1-R
dc.relation.projectIDS2013/MT-3029
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsrestricted access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.cdu544.6
dc.subject.keywordReceptor tyrosine kinase AXL
dc.subject.keywordElectrochemical immunosensor
dc.subject.keywordHuman serum
dc.subject.keywordHeart failure
dc.subject.keywordCancer
dc.subject.ucmQuímica analítica (Química)
dc.subject.unesco2301 Química Analítica
dc.titleElectrochemical immunosensor for receptor tyrosine kinase AXL using poly(pyrrolepropionic acid)-modified disposable electrodes
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number240
dspace.entity.typePublication
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