Vitrification of Large Volumes of Stallion Sperm in Comparison With Spheres and Conventional Freezing: Effect of Warming Procedures and Sperm Selection

Consuegra, Cesar; Crespo Castejón, Francisco; Dorado Martín, Jesús; Díaz Jiménez María; Pereira Aguilar Blasa; Arenas, R; Morrel, J M; Hidalgo Prieto Manuel

Vitrification of Large Volumes of Stallion Sperm in Comparison With Spheres and Conventional Freezing: Effect of Warming Procedures and Sperm Selection

Download

Vitrificaion of Large Volumes of ...pdf (316.69 KB)

Official URL

https://www.sciencedirect.com/journal/journal-of-equine-veterinary-science

Publication date

2019

Authors

Consuegra, Cesar

Crespo Castejón, Francisco

Dorado Martín, Jesús

Díaz Jiménez María

Pereira Aguilar Blasa

Arenas, R

Morrel, J M

Hidalgo Prieto Manuel

Publisher

Elsevier

Citations

Exportar

URI

https://hdl.handle.net/20.500.14352/97196

Citation

10) Consuegra C, Crespo F, Dorado J, Diaz-Jimenez M, Pereira B, Ortiz I, Arenas R, Morrell JM, Hidalgo M. Vitrification of Large Volumes of Stallion Sperm in Comparison With Spheres and Conventional Freezing: Effect of Warming Procedures and Sperm Selection. J Equine Vet Sci. 2019 Dec;83:102680

Abstract

Stallion sperm was vitriﬁed using straws in comparison with spheres and conventional freezing. Vitriﬁcation was performed plunging 30 mL of sperm (spheres) or 0.5 mL straws into liquid nitrogen (LN2) and conventional freezing using 0.5 mL straws frozen in LN2 vapors. Sperm vitriﬁed in straws were submitted to different warming procedures (42􀀀C/20 seconds; 60􀀀C/15 seconds) and single-layer centrifugation (SLC). Total (TM, %) and progressive sperm motility (PM, %), plasma mem-brane (IMS, %) and acrosome integrity (AIS, %) were statistically compared between treatments (mean ± SEM). Signiﬁcant higher values (P < .001) were obtained after vitriﬁcation using spheres in comparison with conventional freezing and vitriﬁcation in straws for TM (54.46 ± 3.2 vs. 36.47 ± 3.2 vs. 2.50 ± 1.2, %), PM (38.63 ± 3.4 vs. 15.11 ± 2.0 vs. 1.9 ± 0.9, %), IMS (65.40 ± 2.8 vs. 50.50 ± 2.8 vs. 21.63 ± 2.1, %), and AIS (48.89 ± 2.8 vs. 15.46 ± 1.7 vs. 4.69 ± 0.9, %). No differences were found between warming procedures. Single-layer centrifugation after warming at 42􀀀C/20 seconds ob-tained higher values (P < .05) than unselected samples for TM (32.52 ± 5.8%), PM (14.22 ± 2.8%), IMS (60.01 ± 3.2%), and AIS (44.5 ± 2.2%), whereas selection after 60􀀀C/15 seconds increased TM (23.11 ± 4.3%) and IMS (67.11 ± 3.9%). In conclusion, vitriﬁcation in spheres obtained better sperm quality than conventional freezing and vitriﬁcation in straws. Warming procedures did not affect the sperm quality but SLC could be a strategy to enhance the quality of the samples after sperm vitriﬁcation using 0.5 mL straws