Vitrification of Large Volumes of Stallion Sperm in Comparison With Spheres and Conventional Freezing: Effect of Warming Procedures and Sperm Selection

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dc.contributor.authorConsuegra, Cesar
dc.contributor.authorCrespo Castejón, Francisco
dc.contributor.authorDorado Martín, Jesús
dc.contributor.authorDíaz Jiménez María
dc.contributor.authorPereira Aguilar Blasa
dc.contributor.authorArenas, R
dc.contributor.authorMorrel, J M
dc.contributor.authorHidalgo Prieto Manuel
dc.date.accessioned2024-01-31T13:19:44Z
dc.date.available2024-01-31T13:19:44Z
dc.date.issued2019-12
dc.description.abstractStallion sperm was vitrified using straws in comparison with spheres and conventional freezing. Vitrification was performed plunging 30 mL of sperm (spheres) or 0.5 mL straws into liquid nitrogen (LN2) and conventional freezing using 0.5 mL straws frozen in LN2 vapors. Sperm vitrified in straws were submitted to different warming procedures (42􀀀C/20 seconds; 60􀀀C/15 seconds) and single-layer centrifugation (SLC). Total (TM, %) and progressive sperm motility (PM, %), plasma mem-brane (IMS, %) and acrosome integrity (AIS, %) were statistically compared between treatments (mean ± SEM). Significant higher values (P < .001) were obtained after vitrification using spheres in comparison with conventional freezing and vitrification in straws for TM (54.46 ± 3.2 vs. 36.47 ± 3.2 vs. 2.50 ± 1.2, %), PM (38.63 ± 3.4 vs. 15.11 ± 2.0 vs. 1.9 ± 0.9, %), IMS (65.40 ± 2.8 vs. 50.50 ± 2.8 vs. 21.63 ± 2.1, %), and AIS (48.89 ± 2.8 vs. 15.46 ± 1.7 vs. 4.69 ± 0.9, %). No differences were found between warming procedures. Single-layer centrifugation after warming at 42􀀀C/20 seconds ob-tained higher values (P < .05) than unselected samples for TM (32.52 ± 5.8%), PM (14.22 ± 2.8%), IMS (60.01 ± 3.2%), and AIS (44.5 ± 2.2%), whereas selection after 60􀀀C/15 seconds increased TM (23.11 ± 4.3%) and IMS (67.11 ± 3.9%). In conclusion, vitrification in spheres obtained better sperm quality than conventional freezing and vitrification in straws. Warming procedures did not affect the sperm quality but SLC could be a strategy to enhance the quality of the samples after sperm vitrification using 0.5 mL straws
dc.description.departmentDepto. de Medicina y Cirugía Animal
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.statuspub
dc.identifier.citation10) Consuegra C, Crespo F, Dorado J, Diaz-Jimenez M, Pereira B, Ortiz I, Arenas R, Morrell JM, Hidalgo M. Vitrification of Large Volumes of Stallion Sperm in Comparison With Spheres and Conventional Freezing: Effect of Warming Procedures and Sperm Selection. J Equine Vet Sci. 2019 Dec;83:102680
dc.identifier.doi10.1016/j.jevs.2019.01.017.
dc.identifier.issn0737-0806
dc.identifier.officialurlhttps://www.sciencedirect.com/journal/journal-of-equine-veterinary-science
dc.identifier.pmid31791531.
dc.identifier.urihttps://hdl.handle.net/20.500.14352/97196
dc.journal.titleJournal Equine Veterinary Science
dc.language.isoeng
dc.page.initial102680
dc.publisherElsevier
dc.relation.projectIDGL-2013-42726-R from Mineco (“Ministerio de Economía Industria y Competitividad”, Spain).
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.cdu636.09
dc.subject.keywordSemen
dc.subject.keywordStallion
dc.subject.keywordVitrification
dc.subject.keywordStraw
dc.subject.keywordSheperes
dc.subject.ucmVeterinaria
dc.subject.unesco24 Ciencias de la Vida
dc.titleVitrification of Large Volumes of Stallion Sperm in Comparison With Spheres and Conventional Freezing: Effect of Warming Procedures and Sperm Selection
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number83
dspace.entity.typePublication
relation.isAuthorOfPublicationd4b46bbe-e6cb-410e-8b74-b2361b41941d
relation.isAuthorOfPublication.latestForDiscoveryd4b46bbe-e6cb-410e-8b74-b2361b41941d
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