Phage-Derived and Aberrant HaloTag Peptides Immobilized on Magnetic Microbeads for Amperometric Biosensing of Serum Autoantibodies and Alzheimer's Disease Diagnosis

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dc.contributor.authorValverde De La Fuente, Alejandro
dc.contributor.authorMontero Calle, Ana
dc.contributor.authorArévalo Pérez, Beatriz
dc.contributor.authorSan Segundo Acosta, Pablo
dc.contributor.authorSerafín González-Carrato, Verónica
dc.contributor.authorAlonso Navarro, Miren
dc.contributor.authorSolís Fernández, Guillermo
dc.contributor.authorPingarrón Carrazón, José Manuel
dc.contributor.authorCampuzano Ruiz, Susana
dc.contributor.authorBarderas Manchado, Rodrigo
dc.date.accessioned2023-06-16T14:19:31Z
dc.date.available2023-06-16T14:19:31Z
dc.date.issued2021-07-27
dc.descriptionCRUE-CSIC (Acuerdos Transformativos 2021)
dc.description.abstractAn electrochemical biosensing platform for serum autoantibodies (AAbs) detection is reported in this work, exploiting for the first time six Alzheimer's disease (AD)-specific phage-derived and frameshift aberrant HaloTag peptides as receptors, immobilized on magnetic microbeads (MBs) surface and captured on disposable electrodes to perform amperometric detection. Operational analytical characteristics and clinical diagnostic ability of the bioplatform were probed in optimized key experimental conditions by analysing serum AAbs of AD patients and healthy subjects. The value of 100% obtained for AUC, sensitivity, and selectivity from the all peptides combined ROC curve, indicate full AD-diagnostic capability of the methodology, which was further implemented, as proof of concept, in a POC multiplexing platform to detect the signature in a single test over clinically actionable times (1 h 15 min), opening great promise for the type of diagnosis and AD patients’ monitoring follow-up currently pursued.
dc.description.departmentDepto. de Química Analítica
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Ciencia e Innovación (MICINN)
dc.description.sponsorshipInstituto de Salud Carlos III (ISCIII)
dc.description.sponsorshipComunidad de Madrid
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/70145
dc.identifier.doidoi.org/10.1002/anse.202100024
dc.identifier.issn2629-2742
dc.identifier.officialurlhttps://doi.org/10.1002/anse.202100024
dc.identifier.urihttps://hdl.handle.net/20.500.14352/4685
dc.journal.titleAnalysis & Sensing
dc.language.isoeng
dc.page.final165
dc.page.initial161
dc.publisherWiley-VCH
dc.relation.projectIDRTI2018-096135-BI00; PID2019-103899RBI00
dc.relation.projectIDPI17CIII/00045 and PI20CIII/00019
dc.relation.projectIDTRANSNANOAVANSENS-CM (S2018/NMT-4349)
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subject.cdu543
dc.subject.keywordAlzheimer’sdisease
dc.subject.keywordautoantibodies
dc.subject.keywordelectrochemical biosensors
dc.subject.keywordphage-derivedpeptides
dc.subject.keywordreceptors
dc.subject.ucmQuímica analítica (Química)
dc.subject.unesco2301 Química Analítica
dc.titlePhage-Derived and Aberrant HaloTag Peptides Immobilized on Magnetic Microbeads for Amperometric Biosensing of Serum Autoantibodies and Alzheimer's Disease Diagnosis
dc.typejournal article
dc.volume.number1
dspace.entity.typePublication
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