Phage-Derived and Aberrant HaloTag Peptides
Immobilized on Magnetic Microbeads for Amperometric
Biosensing of Serum Autoantibodies and Alzheimer's
Disease Diagnosis
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Publication date
2021
Authors
Valverde de la Fuente, Alejandro
Montero Calle, Ana
Alonso Navarro, Miren
Solís Fernández, Guillermo
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Journal Title
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Publisher
Wiley-VCH
Citation
Abstract
An electrochemical biosensing platform for serum autoantibodies (AAbs) detection is reported in this work, exploiting for the first time six Alzheimer's disease (AD)-specific phage-derived and frameshift aberrant HaloTag peptides as receptors, immobilized on magnetic microbeads (MBs) surface and captured on disposable electrodes to perform amperometric detection. Operational analytical characteristics and clinical diagnostic ability of the bioplatform were probed in optimized key experimental conditions by analysing serum AAbs of AD patients and healthy subjects. The value of 100% obtained for AUC, sensitivity, and selectivity from the all peptides combined ROC curve, indicate full AD-diagnostic capability of the methodology, which was further implemented, as proof of concept, in a POC multiplexing platform to detect the signature in a single test over clinically actionable times (1 h 15 min), opening great promise for the type of diagnosis and AD patients’ monitoring follow-up currently pursued.
Description
CRUE-CSIC (Acuerdos Transformativos 2021)