Development of a polarization imaging method to detect paraffin‐embedded pathology tissues before applying other techniques

Sijilmassi, Ouafa; López Alonso, José Manuel; Río Sevilla, Aurora Del; Barrio Asensio, María Del Carmen

Development of a polarization imaging method to detect paraffin‐embedded pathology tissues before applying other techniques

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Ouafa_2020_Wiley_jbio202000288_proof2_.pdf (4.47 MB)

Official URL

https://doi.org/10.1002/jbio.202000288

Publication date

2020

Authors

Sijilmassi, Ouafa

López Alonso, José Manuel

Río Sevilla, Aurora Del

Barrio Asensio, María Del Carmen

Publisher

WILEY‐VCH Verlag GmbH

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URI

https://hdl.handle.net/20.500.14352/6681

Citation

Sijilmassi, O., López Alonso, J. M., Rio Sevilla, A. et al. «Development of a Polarization Imaging Method to Detect Paraffin‐embedded Pathology Tissues before Applying Other Techniques». Journal of Biophotonics, vol. 14, n.o 1, enero de 2021, p. e202000288. DOI.org (Crossref), https://doi.org/10.1002/jbio.202000288.

Abstract

The present article describes the development of a technique, applied to paraffin‐embedded tissues, which uses three different wavelengths of monochromatic light (λ1 = 445 nm, λ2 = 540 nm and λ3 = 660 nm) for the measures of the degree of polarization, degree of linear polarization, degree of circular polarization and birefringence, all obtained from measurements of Stokes parameters by using polarized light. The goal of this study was to detect changes in developing embryonic mouse eye when pregnant mice fed diets without folic acid for variable periods compared with a healthy control group. We present a biomedical diagnostic technique based on polarized light detection applied to paraffin‐embedded tissues to visualize the structural damage to aid us in the diagnosis before applying other techniques. Through this method, we can visualize and identify which parts of the tissue were altered with respect to the control group.