DNA methylation dynamics and MET1a-like gene expression changes during stress-induced pollen reprogramming to embryogenesis

Rodríguez-Serrano, M; Meijón, M; Cañal, MJ; Cifuentes, A; Risueño, MC; Testillano, PS; Solís González, María Teresa

DNA methylation dynamics and MET1a-like gene expression changes during stress-induced pollen reprogramming to embryogenesis

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Official URL

https://pubmed.ncbi.nlm.nih.gov/23175669/

Publication date

2012

Authors

Solís González, María Teresa

Publisher

Journal Experimental Botany

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URI

https://hdl.handle.net/20.500.14352/95739

Citation

olís MT, Rodríguez-Serrano M, Meijón M, Cañal MJ, Cifuentes A, Risueño MC, Testillano PS. DNA methylation dynamics and MET1a-like gene expression changes during stress-induced pollen reprogramming to embryogenesis. J Exp Bot. 2012 Nov;63(18):6431-44. doi: 10.1093/jxb/ers298. PMID: 23175669; PMCID: PMC3504494.

Abstract

Stress-induced plant cell reprogramming involves changes in global genome organization, being the epigenetic modifications key factors in the regulation of genome flexibility. DNA methylation, accomplished by DNA methyltransferases, constitutes a prominent epigenetic modification of the chromatin fibre which is locked in a transcriptionally inactive conformation. Changes in DNA methylation accompany the reorganization of the nuclear architecture during plant cell differentiation and proliferation. After a stress treatment, in vitro-cultured microspores are reprogrammed and change their gametophytic developmental pathway towards embryogenesis, the process constituting a useful system of reprogramming in isolated cells for applied and basic research. Gene expression driven by developmental and stress cues often depends on DNA methylation; however, global DNA methylation and genome-wide expression patterns relationship is still poorly understood. In this work, the dynamics of DNA methylation patterns in relation to nuclear architecture and the expression of BnMET1a-like DNA methyltransferase genes have been analysed during pollen development and pollen reprogramming to embryogenesis in Brassica napus L. by a multidisciplinary approach. Results showed an epigenetic reprogramming after microspore embryogenesis induction which involved a decrease of global DNA methylation and its nuclear redistribution with the change of developmental programme and the activation of cell proliferation, while DNA methylation increases with pollen and embryo differentiation in a cell-type-specific manner. Changes in the presence, abundance, and distribution of BnMET1a-like transcripts highly correlated with variations in DNA methylation. Mature zygotic and pollen embryos presented analogous patterns of DNA methylation and MET1a-like expression, providing new evidence of the similarities between both developmental embryogenic programmes.

Description

This work was supported by Spanish Ministry of Science and Innovation (project BFU2011-23752). M.R.S. was a recipient of a postdoctoral Juan-de-la-Cierva grant (JCI-2007-123-1177). ÍNDICES DE IMPACTO DE LA REVISTA: Citas recibidas • Citas en Sopas: 66 (22-01-2023) • Citas en Web os Science: 63 (28-12-2023) JCR (Journal Impact Factor) • Año 2012 • Factor de impacto de la revista: 5.242 • Factor de impacto sin autocitas: 4.782 • Article influence score: 1.609 • Cuartil mayor: Q1 • Área: PLANT SCIENCES Cuartil: Q1 Posición en el área: 13/197 (Edicion: SCIE) SCImago Journal Rank • Año 2012 • Impacto SJR de la revista: 2.619 • Cuartil mayor: Q1 • Área: Plant Science Cuartil: Q1 Posición en el área: 15/417 • Área: Physiology Cuartil: Q1 Posición en el área: 15/185 Scopus CiteScore • Año 2012 • CiteScore de la revista: 8.5 • Área: Plant Science Percentil: 95 • Área: Physiology Percentil: 89 • Área: Medicine (all) Percentil: 90