DNA methylation dynamics and MET1a-like gene expression changes during stress-induced pollen reprogramming to embryogenesis

Simple item page
dc.contributor.authorRodríguez-Serrano, M
dc.contributor.authorMeijón, M
dc.contributor.authorCañal, MJ
dc.contributor.authorCifuentes, A
dc.contributor.authorRisueño, MC
dc.contributor.authorTestillano, PS
dc.contributor.authorSolís González, María Teresa
dc.date.accessioned2024-01-29T08:44:18Z
dc.date.available2024-01-29T08:44:18Z
dc.date.issued2012
dc.descriptionThis work was supported by Spanish Ministry of Science and Innovation (project BFU2011-23752). M.R.S. was a recipient of a postdoctoral Juan-de-la-Cierva grant (JCI-2007-123-1177). ÍNDICES DE IMPACTO DE LA REVISTA: Citas recibidas • Citas en Sopas: 66 (22-01-2023) • Citas en Web os Science: 63 (28-12-2023) JCR (Journal Impact Factor) • Año 2012 • Factor de impacto de la revista: 5.242 • Factor de impacto sin autocitas: 4.782 • Article influence score: 1.609 • Cuartil mayor: Q1 • Área: PLANT SCIENCES Cuartil: Q1 Posición en el área: 13/197 (Edicion: SCIE) SCImago Journal Rank • Año 2012 • Impacto SJR de la revista: 2.619 • Cuartil mayor: Q1 • Área: Plant Science Cuartil: Q1 Posición en el área: 15/417 • Área: Physiology Cuartil: Q1 Posición en el área: 15/185 Scopus CiteScore • Año 2012 • CiteScore de la revista: 8.5 • Área: Plant Science Percentil: 95 • Área: Physiology Percentil: 89 • Área: Medicine (all) Percentil: 90
dc.description.abstractStress-induced plant cell reprogramming involves changes in global genome organization, being the epigenetic modifications key factors in the regulation of genome flexibility. DNA methylation, accomplished by DNA methyltransferases, constitutes a prominent epigenetic modification of the chromatin fibre which is locked in a transcriptionally inactive conformation. Changes in DNA methylation accompany the reorganization of the nuclear architecture during plant cell differentiation and proliferation. After a stress treatment, in vitro-cultured microspores are reprogrammed and change their gametophytic developmental pathway towards embryogenesis, the process constituting a useful system of reprogramming in isolated cells for applied and basic research. Gene expression driven by developmental and stress cues often depends on DNA methylation; however, global DNA methylation and genome-wide expression patterns relationship is still poorly understood. In this work, the dynamics of DNA methylation patterns in relation to nuclear architecture and the expression of BnMET1a-like DNA methyltransferase genes have been analysed during pollen development and pollen reprogramming to embryogenesis in Brassica napus L. by a multidisciplinary approach. Results showed an epigenetic reprogramming after microspore embryogenesis induction which involved a decrease of global DNA methylation and its nuclear redistribution with the change of developmental programme and the activation of cell proliferation, while DNA methylation increases with pollen and embryo differentiation in a cell-type-specific manner. Changes in the presence, abundance, and distribution of BnMET1a-like transcripts highly correlated with variations in DNA methylation. Mature zygotic and pollen embryos presented analogous patterns of DNA methylation and MET1a-like expression, providing new evidence of the similarities between both developmental embryogenic programmes.
dc.description.departmentDepto. de Genética, Fisiología y Microbiología
dc.description.facultyFac. de Ciencias Biológicas
dc.description.refereedTRUE
dc.description.statuspub
dc.identifier.citationolís MT, Rodríguez-Serrano M, Meijón M, Cañal MJ, Cifuentes A, Risueño MC, Testillano PS. DNA methylation dynamics and MET1a-like gene expression changes during stress-induced pollen reprogramming to embryogenesis. J Exp Bot. 2012 Nov;63(18):6431-44. doi: 10.1093/jxb/ers298. PMID: 23175669; PMCID: PMC3504494.
dc.identifier.doi10.1093/JXB/ERS298
dc.identifier.issn0022-0957
dc.identifier.officialurlhttps://pubmed.ncbi.nlm.nih.gov/23175669/
dc.identifier.pmid23175669
dc.identifier.urihttps://hdl.handle.net/20.500.14352/95739
dc.issue.number18
dc.journal.titleJournal of Experimental Botany
dc.language.isoeng
dc.page.final6444
dc.page.initial6431
dc.publisherJournal Experimental Botany
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsopen access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.cdu581.15
dc.subject.keywordCell fate, differentiation, DNA methyltransferase, epigenetics, male gametophyte development, 5-methylcytosine, microspore culture, pollen embryogenesis, proliferation
dc.subject.ucmBiología celular (Biología)
dc.subject.ucmBiotecnología
dc.subject.unesco31 Ciencias Agrarias
dc.subject.unesco2417.12 Citología Vegetal
dc.titleDNA methylation dynamics and MET1a-like gene expression changes during stress-induced pollen reprogramming to embryogenesis
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number63
dspace.entity.typePublication
relation.isAuthorOfPublication3da5e099-3087-4d19-8ed0-60a12692e004
relation.isAuthorOfPublication.latestForDiscovery3da5e099-3087-4d19-8ed0-60a12692e004
Download
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Solís MT. 2012_Aportación 2-Tramo 1.pdf
Size:
3.36 MB
Format:
Adobe Portable Document Format
downoload Download
Collections
Artículos