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Epitope mapping of the major allergen from Atlantic cod in Spanish population reveals different IgE‐binding patterns

dc.contributor.authorPerez‐Gordo, Marina
dc.contributor.authorPastor Vargas, Carlos
dc.contributor.authorJing Lin
dc.contributor.authorBardina, Ludmilla
dc.contributor.authorCases, Barbara
dc.contributor.authorIbáñez, Maria Dolores
dc.contributor.authorVivanco Martínez, Fernando
dc.contributor.authorCuesta‐Herranz, Javier
dc.contributor.authorSampson, Hugh
dc.date.accessioned2024-01-22T12:09:11Z
dc.date.available2024-01-22T12:09:11Z
dc.date.issued2013
dc.description.abstractScope: IgE-epitope mapping of allergens reveal important information about antigen components involved in allergic reactions. The peptide-based microarray immunoassay has been used to map epitopes of some food allergens. We developed a peptide microarray immunoassay to map allergenic epitopes in parvalbumin from Atlantic cod (Gad m 1), the most consumed cod species in Spain. Methods and results: Sera from 13 fish-allergic patients with specific IgE to cod parvalbumin were used. A library of overlapping peptides was synthesized, representing the primary sequence of Gad m 1. Peptides were used to analyze allergen-specific IgE antibodies in patient sera. 100% of the patients recognized one antigenic region of 15 amino acids in length in Gad m 1. This region only partially correlated with one of the three antigenic determinants of Gad c 1 (Allergen M), parvalbumin from Baltic cod (Gadus callarias). In the 3D model of the protein, this region was located on the surface of the protein. Conclusion: We have identified a relevant antigenic region in Gad m 1. This epitope could be considered as a severity marker and provides additional information to improve fish allergy diagnosis and the design of safe immunotherapeutic tools.
dc.description.departmentDepto. de Bioquímica y Biología Molecular
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipMinisterio de Educación y Ciencia (España)
dc.description.statuspub
dc.identifier.citationPerez-Gordo M, Pastor-Vargas C, Lin J, Bardina L, Cases B, Ibáñez MD, Vivanco F, Cuesta-Herranz J, Sampson HA. Epitope mapping of the major allergen from Atlantic cod in Spanish population reveals different IgE-binding patterns. Mol Nutr Food Res. 2013 Jul;57(7):1283-90. doi: 10.1002/mnfr.201200332. Epub 2013 Apr 2. PMID: 23554100.
dc.identifier.doi10.1002/mnfr.201200332
dc.identifier.essn1613-4133
dc.identifier.issn1613-4125
dc.identifier.officialurlhttps://doi.org/10.1002/mnfr.201200332
dc.identifier.urihttps://hdl.handle.net/20.500.14352/94354
dc.issue.number7
dc.journal.titleMolecular Nutrition Food Research
dc.language.isoeng
dc.page.final1290
dc.page.initial1283
dc.publisherWiley
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.accessRightsrestricted access
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.cdu577.1
dc.subject.keywordCod parvalbumin
dc.subject.keywordEpitope mapping
dc.subject.keywordFish allergy
dc.subject.keywordFood allergy
dc.subject.ucmAlergología
dc.subject.unesco2403 Bioquímica
dc.titleEpitope mapping of the major allergen from Atlantic cod in Spanish population reveals different IgE‐binding patterns
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number57
dspace.entity.typePublication
relation.isAuthorOfPublication25af78c7-0077-4891-a14e-bcd8e51fe408
relation.isAuthorOfPublication6f3e7679-cbc7-4f23-8355-2de0876d46ad
relation.isAuthorOfPublication.latestForDiscovery25af78c7-0077-4891-a14e-bcd8e51fe408

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