Epitope mapping of the major allergen from Atlantic cod in Spanish population reveals different IgE‐binding patterns
dc.contributor.author | Perez‐Gordo, Marina | |
dc.contributor.author | Pastor Vargas, Carlos | |
dc.contributor.author | Jing Lin | |
dc.contributor.author | Bardina, Ludmilla | |
dc.contributor.author | Cases, Barbara | |
dc.contributor.author | Ibáñez, Maria Dolores | |
dc.contributor.author | Vivanco Martínez, Fernando | |
dc.contributor.author | Cuesta‐Herranz, Javier | |
dc.contributor.author | Sampson, Hugh | |
dc.date.accessioned | 2024-01-22T12:09:11Z | |
dc.date.available | 2024-01-22T12:09:11Z | |
dc.date.issued | 2013 | |
dc.description.abstract | Scope: IgE-epitope mapping of allergens reveal important information about antigen components involved in allergic reactions. The peptide-based microarray immunoassay has been used to map epitopes of some food allergens. We developed a peptide microarray immunoassay to map allergenic epitopes in parvalbumin from Atlantic cod (Gad m 1), the most consumed cod species in Spain. Methods and results: Sera from 13 fish-allergic patients with specific IgE to cod parvalbumin were used. A library of overlapping peptides was synthesized, representing the primary sequence of Gad m 1. Peptides were used to analyze allergen-specific IgE antibodies in patient sera. 100% of the patients recognized one antigenic region of 15 amino acids in length in Gad m 1. This region only partially correlated with one of the three antigenic determinants of Gad c 1 (Allergen M), parvalbumin from Baltic cod (Gadus callarias). In the 3D model of the protein, this region was located on the surface of the protein. Conclusion: We have identified a relevant antigenic region in Gad m 1. This epitope could be considered as a severity marker and provides additional information to improve fish allergy diagnosis and the design of safe immunotherapeutic tools. | |
dc.description.department | Depto. de Bioquímica y Biología Molecular | |
dc.description.faculty | Fac. de Ciencias Químicas | |
dc.description.refereed | TRUE | |
dc.description.sponsorship | Ministerio de Educación y Ciencia (España) | |
dc.description.status | pub | |
dc.identifier.citation | Perez-Gordo M, Pastor-Vargas C, Lin J, Bardina L, Cases B, Ibáñez MD, Vivanco F, Cuesta-Herranz J, Sampson HA. Epitope mapping of the major allergen from Atlantic cod in Spanish population reveals different IgE-binding patterns. Mol Nutr Food Res. 2013 Jul;57(7):1283-90. doi: 10.1002/mnfr.201200332. Epub 2013 Apr 2. PMID: 23554100. | |
dc.identifier.doi | 10.1002/mnfr.201200332 | |
dc.identifier.essn | 1613-4133 | |
dc.identifier.issn | 1613-4125 | |
dc.identifier.officialurl | https://doi.org/10.1002/mnfr.201200332 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14352/94354 | |
dc.issue.number | 7 | |
dc.journal.title | Molecular Nutrition Food Research | |
dc.language.iso | eng | |
dc.page.final | 1290 | |
dc.page.initial | 1283 | |
dc.publisher | Wiley | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | en |
dc.rights.accessRights | restricted access | |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.subject.cdu | 577.1 | |
dc.subject.keyword | Cod parvalbumin | |
dc.subject.keyword | Epitope mapping | |
dc.subject.keyword | Fish allergy | |
dc.subject.keyword | Food allergy | |
dc.subject.ucm | Alergología | |
dc.subject.unesco | 2403 Bioquímica | |
dc.title | Epitope mapping of the major allergen from Atlantic cod in Spanish population reveals different IgE‐binding patterns | |
dc.type | journal article | |
dc.type.hasVersion | VoR | |
dc.volume.number | 57 | |
dspace.entity.type | Publication | |
relation.isAuthorOfPublication | 25af78c7-0077-4891-a14e-bcd8e51fe408 | |
relation.isAuthorOfPublication | 6f3e7679-cbc7-4f23-8355-2de0876d46ad | |
relation.isAuthorOfPublication.latestForDiscovery | 25af78c7-0077-4891-a14e-bcd8e51fe408 |
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