Epitope mapping of the major allergen from Atlantic cod in Spanish population reveals different IgE‐binding patterns
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2013
Authors
Perez‐Gordo, Marina
Jing Lin
Bardina, Ludmilla
Cases, Barbara
Ibáñez, Maria Dolores
Cuesta‐Herranz, Javier
Sampson, Hugh
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Wiley
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Perez-Gordo M, Pastor-Vargas C, Lin J, Bardina L, Cases B, Ibáñez MD, Vivanco F, Cuesta-Herranz J, Sampson HA. Epitope mapping of the major allergen from Atlantic cod in Spanish population reveals different IgE-binding patterns. Mol Nutr Food Res. 2013 Jul;57(7):1283-90. doi: 10.1002/mnfr.201200332. Epub 2013 Apr 2. PMID: 23554100.
Abstract
Scope: IgE-epitope mapping of allergens reveal important information about antigen components involved in allergic reactions. The peptide-based microarray immunoassay has been used to map epitopes of some food allergens. We developed a peptide microarray immunoassay to map allergenic epitopes in parvalbumin from Atlantic cod (Gad m 1), the most consumed cod species in Spain.
Methods and results: Sera from 13 fish-allergic patients with specific IgE to cod parvalbumin were used. A library of overlapping peptides was synthesized, representing the primary sequence of Gad m 1. Peptides were used to analyze allergen-specific IgE antibodies in patient sera. 100% of the patients recognized one antigenic region of 15 amino acids in length in Gad m 1. This region only partially correlated with one of the three antigenic determinants of Gad c 1 (Allergen M), parvalbumin from Baltic cod (Gadus callarias). In the 3D model of the protein, this region was located on the surface of the protein.
Conclusion: We have identified a relevant antigenic region in Gad m 1. This epitope could be considered as a severity marker and provides additional information to improve fish allergy diagnosis and the design of safe immunotherapeutic tools.