Bioactive glass as precursor of designed‐architecture scaffolds for tissue engineering
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2006
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Wiley Periodicals, Inc.
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Padilla S, Sánchez‐Salcedo S, Vallet‐Regí M. Bioactive glass as precursor of designed‐architecture scaffolds for tissue engineering. J Biomedical Materials Res 2007;81A:224–32. https://doi.org/10.1002/jbm.a.30934
Abstract
In this work, the conditions to obtain concentrated and fluid suspensions from a bioactive glass (55-SiO2; 41-CaO; 4-P2O5; mol %) were investigated. The influence of the heat treatment of the glass on the specific surface area, solubility, bioactivity, and finally on their dispersion characteristics was studied. Zeta potential and viscosity measurements were carried out, and based on the obtained results, the best dispersant was selected. The optimum concentration of dispersant, maximum content of solid and time of mixing were also investigated. Slurries containing 50 vol % could be obtained calcining the glass at 1100°C and using Darvan 811 (sodium polyacrylate) as dispersant. Scaffolds with designed architecture were prepared from these suspensions combining the gelcasting method and the stereolithography technique. A polymeric negative (replica of the desired structure) was previously obtained by stereolithography. The slurry was cast into the molds and then polymerized (gelcasting method). The negative was eliminated by heat treatment. After sintering at 1300°C, scaffolds with interconnected porosity and three-dimensional channels of 400–470 μm and macropores of 1.4 μm were obtained.
Description
En este trabajo se investigaron las condiciones para obtener suspensiones concentradas y fluidas de un vidrio bioactivo (55-SiO2; 41-CaO; 4-P2O5; mol %). Se estudió la influencia del tratamiento térmico del vidrio sobre la superficie específica, la solubilidad, la bioactividad y, finalmente, sobre la dispersión de las partículas. Se llevaron a cabo mediciones del potencial zeta y de la viscosidad y, en función de los resultados obtenidos, se seleccionó el mejor dispersante. También se investigó la concentración óptima de dispersante, el contenido máximo de sólido y el tiempo de mezcla. Se pudieron obtener suspensiones con un contenido del 50 % vol calcinando el vidrio a 1100°C y utilizando Darvan 811 (poliacrilato sódico) como dispersante. A partir de estas suspensiones se prepararon andamiajes con la arquitectura diseñada combinando el método de gelcasting y la técnica de estereolitografía. Previamente se obtuvo un negativo polimérico (réplica de la estructura deseada) mediante estereolitografía. La suspensión se vació en los moldes y después se polimerizó (método gelcasting). El negativo se eliminó mediante tratamiento térmico. Tras la sinterización a 1300 °C, se obtuvieron andamiajes con porosidad interconectada y canales tridimensionales de 400-470 μm y macroporos de 1,4 μm.