Watermelon Profilin: Characterization of a Major Allergen as a Model for Plant-Derived Food Profilins
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2010
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Karger
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Cases B, Pastor-Vargas C, Dones FG, Perez-Gordo M, Maroto AS, de las Heras M, Vivanco F, Cuesta-Herranz J. Watermelon profilin: characterization of a major allergen as a model for plant-derived food profilins. Int Arch Allergy Immunol. 2010;153(3):215-22. doi: 10.1159/000314361. Epub 2010 May 18. PMID: 20484919.
Abstract
Background: Plant profilins have been reported as minor allergens. They are a well-known pan-allergen family responsible for cross-reactivity between plant-derived foods and pollens. Watermelon profilin has been reported to be a major allergen in watermelon (Citrullus lanatus).The aim of this study was to characterize recombinant watermelon profilin, confirming its reactivity for diagnostic purposes and the development of immunotherapy.
Methods: Native profilin was purified from watermelon extract by affinity chromatography using poly-L-proline. Recombinant His-tagged profilin was produced in Pichia pastoris yeast using pPICZαA vector and purified by metal chelate affinity chromatography. ELISA and immunoblot were carried out with sera from 17 watermelon-allergic patients. Biological activity was tested by the basophil activation test.
Results: Native profilin and recombinant profilin were purified and identified by mass spectrometry. Both show similar IgE reactivity in vitro and are biologically active.
Conclusions: Similarities were found in the IgE-binding patterns and biological activity of recombinant profilin and native profilin. Recombinant profilin may be a powerful tool for specific diagnosis.