Enhancement of DNA vaccine potency through linkage of antigen to filamentous bacteriophage coat protein III domain I

Cuesta Martínez, Ángel; Suárez Porto, Eduardo; Larsen, Martin; Jensen, Kim Bak; Sanz, Laura; Compte Grau, Marta; Kristensen, Peter; Álvarez‐Vallina, Luis

Enhancement of DNA vaccine potency through linkage of antigen to filamentous bacteriophage coat protein III domain I

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Official URL

https://doi.org/10.1111/j.1365-2567.2006.02325.x

Publication date

2006

Authors

Cuesta Martínez, Ángel

Suárez Porto, Eduardo

Álvarez‐Vallina, Luis

Publisher

Wiley

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URI

https://hdl.handle.net/20.500.14352/101153

Citation

Cuesta AM, Suárez E, Larsen M, et al. Enhancement of DNA vaccine potency through linkage of antigen to filamentous bacteriophage coat protein III domain I. Immunology. 2006;117(4):502-506. doi:10.1111/j.1365-2567.2006.02325.x

Abstract

Although DNA‐based cancer vaccines have been successfully tested in mouse models, a major drawback of cancer vaccination still remains, namely that tumour antigens are weak and fail to generate a vigorous immune response in tumour‐bearing patients. Genetic technology offers strategies for promoting immune pathways by adding immune‐activating genes to the tumour antigen sequence. In this work, we converted a model non‐immunogenic antigen into a vaccine by fusing it to domain I of the filamentous bacteriophage coat protein III gene. Vaccination with a DNA construct encoding the domain I fusion generated antigen‐specific T helper 1‐type cellular immune responses. These results demonstrate that the incorporation of protein III into a DNA vaccine formulation can modulate the gene‐mediated immune response and may thus provide a strategy for improving its therapeutic effect.