Person:
Sánchez-Paniagua López, Marta

Profile Picture
First Name
Marta
Last Name
Sánchez-Paniagua López
URI
https://hdl.handle.net/20.500.14352/77530
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Farmacia
Department
Química en Ciencias Farmacéuticas
Area
Química Analítica
Identifiers
UCM identifierORCIDScopus Author IDWeb of Science ResearcherIDDialnet ID

Filters

Reset filters

Settings

Search Results

Now showing 1 - 10 of 22
  • Thumbnail Image
    Item
    Electrochemical genosensor for Klotho detection based on aliphatic and aromatic thiols self-assembled monolayers
    (Talanta, 2020) Sánchez-Paniagua López, Marta; Palenzuela Batista, Sara; Manzanares Palenzuela, Lorena; López Ruiz, María Beatriz
    Changes in expression of Klotho gene are associated with chronic kidney disease and its potential as early biomarker is being studied. We report, for the first time, the detection of Klotho gene by a biosensor platform. Self-assembled mixed monolayers (SAMs) as DNA immobilization method in screen-printed gold electrodes and a sandwich format detection were used in the development of an electrochemical genosensor for the detection of a 100-mer DNA fragment, copy of the partial region of the mRNA Klotho gene. The use of different binary and ternary SAMs based on aliphatic (mercaptohexanol, MCH, and hexanedithiol, HDT) and aromatic (mercaptophenylacetic acid, MPAA) thiol diluents and capture probe (CP) as sensing phases was evaluated by cyclic voltammetry and electrochemical impedance spectroscopy. Multiple configurations were studied, changing the order of component addition and comparing co-immobilization and two-step immobilization processes. The procedure for binary SAM preparation consisting of sequential addition of a thiol diluent followed by CP was found to have the least detrimental impact on electrochemical performance. The signal-to-blank ratios increased considerably in the case of thioaromatic binary DNA monolayers, MPPA/CP, compared to the values obtained for aliphatic SAMs. Ternary monolayers formed by MCH and HDT rendered good fractional coverage levels and generated more reversible redox reactions at the surface, mostly when CP was firstly immobilized, CP/HDT/MCH. A significant reduction of the blank and non-specific (non-complementary sequence) signals was obtained with this ternary SAM, compared to binary SAMs and an increase of 2.42-fold of the S/B ratio (10 nM of target) compared with MPAA/CP SAMs. A linear response in the range of 5·10−10 to 5·10−8 M was obtained with CP/HDT/MCH monolayer, with a detection limit of 0.5 nM and RSD of 8.10%.
  • Thumbnail Image
    Item
    Project number: 185
    ¿Y si nos escapamos de manera virtual? Hacia la virtualización de las "escape rooms"
    (2021) Mateos-Aparicio Cediel, Inmaculada; Sánchez-Paniagua López, Marta; López Ruiz, María Beatriz; Rueda Rodríguez, María del Carmen; García Alonso, María Alejandra; Molina Santos, Marina Mercedes; Moreno Guzmán, María; Pérez Rodríguez, María Luisa; Raggio Quilez, José; Sevilla Sierra, María Paz; González Aguilera, Guillermo; Gonzalez de Toro, Teresa Almudena; Jiménez de la Peña, Rocío Teresa
  • Thumbnail Image
    Item
    Edible plant by-products as source of polyphenols: prebiotic effect and analytical methods
    (Critical Reviews in Food Science and Nutrition, 2022) García Alonso, María Alejandra; Sánchez-Paniagua López, Marta; Manzanares-Palenzuela, C. Lorena; Redondo Cuenca, Araceli; López Ruiz, María Beatriz
    Polyphenols with high chemical diversity are present in vegetables both in the edible parts and by-products. A large proportion of them remains unabsorbed along the gastrointestinal tract, being accumulated in the colon, where they are metabolized by the intestinal microbiota. These polyphenols have been found to have “prebiotic-like” effects. The edible plant industry generates tons of residues called by-products, which consist of unutilized plant tissues (peels, husks, calyxes and seeds). Their disposal requires special and costly treatments to avoid environmental complications. Reintroducing these by-products into the value chain using technological and biotechnological practices is highly appealing since many of them contain nutrients and bioactive compounds, such as polyphenols, with many health-promoting properties. Edible plant by-products as a source of polyphenols highlights the need for analytical methods. Analytical methods are becoming increasingly selective, sensitive and precise, but the great breakthrough lies in the pretreatment of the sample and in particular in the extraction methods. This review shows the importance of edible plant by-products as a source of polyphenols, due to their prebiotic effect, and to compile the most appropriate analytical methods for the determination of the total content of phenolic compounds as well as the detection and quantification of individual polyphenols.
  • Thumbnail Image
    Item
    Biosensores amperométricos de tirosinasa para la determinación de compuestos fenólicos en medios acuosos y no acuosos
    (2009) Sánchez-Paniagua López, Marta; López Ruiz, Beatriz; López Cabarcos, Enrique
  • Thumbnail Image
    Item
    The Pharmacological Activity of Camellia sinensis (L.) Kuntze on Metabolic and Endocrine Disorders: A Systematic Review
    (Biomolecules, 2020) Sánchez-Paniagua López, Marta; González Burgos, Elena María; Iglesias Peinado, Irene; Lozano Fernández, Rafael; Gómez-Serranillos Cuadrado, María Pilar
    Tea made from Camellia sinensis leaves is one of the most consumed beverages worldwide. This systematic review aims to update Camellia sinensis pharmacological activity on metabolic and endocrine disorders. Inclusion criteria were preclinical and clinical studies of tea extracts and isolated compounds on osteoporosis, hypertension, diabetes, metabolic syndrome, hypercholesterolemia, and obesity written in English between 2014 and 2019 and published in Pubmed, Science Direct, and Scopus. From a total of 1384 studies, 80 reports met inclusion criteria. Most papers were published in 2015 (29.3%) and 2017 (20.6%), conducted in China (28.75%), US (12.5%), and South Korea (10%) and carried out with extracts (67.5%, especially green tea) and isolated compounds (41.25%, especially epigallocatechin gallate). Most pharmacological studies were in vitro and in vivo studies focused on diabetes and obesity. Clinical trials, although they have demonstrated promising results, are very limited. Future research should be aimed at providing more clinical evidence on less studied pathologies such as osteoporosis, hypertension, and metabolic syndrome. Given the close relationship among all endocrine disorders, it would be of interest to find a standard dose of tea or their bioactive constituents that would be beneficial for all of them.
  • Thumbnail Image
    Item
    Biosensors for GMO Testing: Nearly 25 Years of Research
    (2018) Sánchez-Paniagua López, Marta; Manzanares Palenzuela, Carmen Lorena ; López Ruiz, María Beatriz
    In the nearly two decades since genetically modified organisms (GMOs) were first commercialized, genetically engineered crops have gained ground on their conventional counterparts, reaching 185 million hectares worldwide in 2016. The technology has bestowed most of its benefits on enhancing crop productivity with two main traits currently dominating the market: insect-resistant and herbicide-tolerant crops. Despite their rapid and vast adoption by farmers worldwide, GMOs have generated heated debates, especially in European countries (EU), driven mostly by consumers concerned about safety of transgenic foods and about the potential impact on the environment. The need to monitor and to verify the presence and the amount of GMOs in agricultural crops and in food products has generated interest in analytical methods for sensitive, accurate, rapid, and cheap detection of these products. DNA biosensors have been envisioned as a novel DNA-detection technology that would one day substitute current amplification-based methods, providing hand-held, quick, and ultrasensitive gene-level detection. This review summarizes the contributions made in nearly 20 years of research regarding the application of genosensing technology for the qualitative and quantitative determination of transgenic traits.
  • Thumbnail Image
    Item
    Project number: 366
    Indicadores de calidad para evaluar diferentes estrategias metodológicas implantadas en la docencia práctica del Grado de Farmacia
    (2016) Hervás Pérez, Juan Pablo; Sánchez-Paniagua López, Marta; Rodríguez Rodríguez, Elena; Ródenas de la Rocha, Sofía; Martín Gómez, Mª Carmen
  • Thumbnail Image
    Item
    Project number: 358
    Estrategias metodológicas para la adquisición de competencias en la docencia práctica de química analítica. Evaluación mediante indicadores de calidad
    (2015) Sánchez-Paniagua López, Marta; Hérvas Pérez, Juan Pablo; Rodríguez-Rodriguez, Elena; Martín-Fernandez, Begoña; Manzanares Palenzuela, C. Lorena
    Nuevas estrategias metodológicas en la adquisición y desarrollo de competencias por los estudiantes en el laboratorio de Química Analítica. Seguimiento y evaluación mediante indicadores de calidad para la mejora continua de la práctica docente
  • Thumbnail Image
    Item
    Synthesis, characterization and use of enzyme cashew gum nanoparticles for biosensing applications
    (Journal of Materials Chemistry B, 2021) Amorim, Adriany das Graças Nascimento; Sánchez-Paniagua López, Marta; Oliveira, Taiane Maria de; Mafud, Ana Carolina; Alves da Silva, Durcilene; Souza de Almeida Leite, José Roberto de; López Ruiz, María Beatriz
    This research reports, for the first time, the immobilization of an enzyme - Rhus vernificera laccase - on cashew gum (CG) nanoparticles (NPs) and its application as a biological layer in the design and development of an electrochemical biosensor. Laccase-CG nanoparticles (LacCG-NPs) were prepared by the nanoprecipitation method and characterized by UV-Vis spectrophotometry, atomic force microscopy, scanning electron microscopy, attenuated total reflectance-Fourier-transform infrared spectroscopy, circular dichroism, cyclic voltammetry, and electrochemical impedance spectroscopy. The average size and stability of the NPs were predicted by DLS and zeta potential. The ATR-FTIR results clearly demonstrated an interaction between –NH and –OH groups to form LacCG-NPs. The average size found for LacCG-NPs was 280 53 nm and a polydispersity index of 0.309 0.08 indicated a good particle size distribution. The zeta potential shows a good colloidal stability. The use of a natural product to prepare the enzymatic nanoparticles, its easy synthesis and the immobilization efficiency should be highlighted. LacCG-NPs were successfully applied as a biolayer in the development of an amperometric biosensor for catechol detection. The resulting device showed a low response time (6 s), good sensitivity (7.86 mA mM1 cm2 ), wide linear range of 2.5 107 –2.0 104 M, and low detection limit (50 nM).
  • Thumbnail Image
    Item
    Project number: 118
    "Escape Seminar": Escápate del seminario
    (2020) Mateos-Aparicio Cediel, Inmaculada; Sánchez-Paniagua López, Marta; García Alonso, María Alejandra; López Ruiz, María Beatriz; Molina Santos, Marina Mercedes; Moreno Guzmán, María; Raggio Quilez, José; Rueda, María del Carmen; Sevilla Sierra, María Paz; Bodas Folguera, Cristina; Jimenez de la Peña Armada, Rocio Teresa
    Las "escape room" son juegos de equipo para "escapar" de una sala o una sala virtual. Esta sala está llena de desafíos que se deben superar para ganar (escapar) dentro de un límite de tiempo determinado. Debido a que la enseñanza tradicional está causando problemas en torno a la motivación y el compromiso, y muchos estudiantes la perciben como ineficaz y aburrida, el uso de juegos educativos es muy prometedor porque son una herramienta de enseñanza para mejorar las habilidades no cognitivas, como la resolución de problemas y la colaboración. y comunicación. Los juegos tienen un notable poder motivador debido al uso de una serie de mecanismos que alientan a las personas a participar en ellos, a menudo sin ninguna recompensa, solo por la satisfacción de jugar y la posibilidad de ganar. En el curso 2019-2020, hemos desarrollado actividades basadas en el concepto de la "escape room" en las asignaturas: "Ciencia y Análisis de agua y bebidas" del Grado en Ciencia y Tecnología de Alimentos, "Química Analítica I" y "Físico-Química Farmacéutica" del Grado de Farmacia y “Química” del Grado en Óptica y Optometría de la Universidad Complutense de Madrid. Para el desarrollo del proyecto, todas las características y el progreso de las diferentes fases del seminario de la "escape room" se informaron en el primer día de clase y a través de la plataforma del Campus Virtual. Los estudiantes se agruparon (5-7 personas / grupo) y nombraron un portavoz que fue responsable de responder los retos de la actividad. Al final de esta acción, los estudiantes respondieron voluntariamente a las encuestas Likert para evaluar sus implicaciones y su percepción sobre su aprendizaje, trabajo en equipo y si les gustó la actividad propuesta.