Wire Myography to Study Vascular Tone and Vascular Structure of Isolated Mouse Arteries
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2015
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Springer Science+Business Media
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del Campo L, Ferrer M. Wire Myography to Study Vascular Tone and Vascular Structure of Isolated Mouse Arteries. Methods Mol Biol. 2015;1339:255-76
Del Campo L, Ferrer M (2015) Wire Myography to Study Vascular Tone and Vascular Structure of Isolated Mouse Arteries. In: Andrés V, Dorado B (eds) Methods in Mouse Atherosclerosis. Springer New York, New York, NY, pp 255–276
Del Campo L, Ferrer M (2015) Wire Myography to Study Vascular Tone and Vascular Structure of Isolated Mouse Arteries. In: Andrés V, Dorado B (eds) Methods in Mouse Atherosclerosis. Springer New York, New York, NY, pp 255–276
Abstract
Atherosclerosis is characterized by endothelial dysfunction and alterations in vascular reactivity, which can be investigated by wire myography. The method allows ex vivo monitoring of the transversal isometric tension developed by a vessel segment in response to different pathophysiological stimuli. Here we describe in detail how to use the wire myograph to evaluate endothelial function and vasoconstrictor or vasodilator properties of the vessel, as well as to identify and characterize different factors and molecular pathways that control vascular tone. We also describe how to use the wire myograph to analyze biomechanical and passive properties of vessels such as diameter and elasticity.