Minimal Residual Disease Monitoring with Next-Generation Sequencing Methodologies in Hematological Malignancies

Sánchez, Ricardo; Ayala Díaz, Rosa María; Martínez López, Joaquín

Minimal Residual Disease Monitoring with Next-Generation Sequencing Methodologies in Hematological Malignancies

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ijms-20-02832-v2.pdf (598.78 KB)

Official URL

https://doi.org/10.3390/ijms20112832

Publication date

2019

Authors

Sánchez, Ricardo

Ayala Díaz, Rosa María

Martínez López, Joaquín

Publisher

MDPI

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URI

https://hdl.handle.net/20.500.14352/12572

Abstract

Ultra-deep next-generation sequencing has emerged in recent years as an important diagnostic tool for the detection and follow-up of tumor burden in most of the known hematopoietic malignancies. Meticulous and high-throughput methods for the lowest possible quantified disease are needed to address the deficiencies of more classical techniques. Precision-based approaches will allow us to correctly stratify each patient based on the minimal residual disease (MRD) after a treatment cycle. In this review, we consider the most prominent ways to approach next-generation sequencing methodologies to follow-up MRD in hematological neoplasms.