Preparation of Lipase-Coated, Stabilized, Hydrophobic Magnetic Particles for Reversible Conjugation of Biomacromolecules

Abstract

This Communication presents the developmentof a novel strategy for the easy conjugation of biomolecules tohydrophobic magnetic microparticles via reversible coating withpreviously functionalized lipase molecules. First, the ability oflipase to be strongly adsorbed onto hydrophobic surfaces wasexploited for the stabilization of microparticles in aqueousmedium by the creation of a hydrophilic surface. Second, thesurface amino acids of lipase can be tailored to suit biomoleculeconjugation. This approach has been demonstrated by amino-epoxy activation of lipase, enabling the conjugation of differentbiomolecules to the magnetic particle’s surface. For example, itwas possible to immobilize 70% ofEscherichia coliproteins onthe recovered particles. Furthermore, this strategy could beextended to other lipase chemical modification protocols,enablingfine control of biomolecule coupling. These conjugation techniques constitute a modular methodology that also permitsthe recycling of the magnetic carrier following use