Simultaneous determination of zearalenone and alternariol mycotoxins in oil samples using mixed molecularly imprinted polymer beads

Moya-Cavas, Tamara; Navarro Villoslada, Fernando; Urraca Ruiz, Javier; Antonio Serrano, Luis; Orellana Moraleda, Guillermo; Moreno-Bondi, María Cruz; Moreno Bondi, María Cruz

Simultaneous determination of zearalenone and alternariol mycotoxins in oil samples using mixed molecularly imprinted polymer beads

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Official URL

https://doi.org/10.1016/j.foodchem.2023.135538

Publication date

2023

Authors

Moya-Cavas, Tamara

Navarro Villoslada, Fernando

Urraca Ruiz, Javier

Antonio Serrano, Luis

Orellana Moraleda, Guillermo

Moreno-Bondi, María Cruz

Moreno Bondi, María Cruz

Publisher

Elsevier

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URI

https://hdl.handle.net/20.500.14352/91822

Citation

Moya-Cavas, T.; Navarro-Villoslada, F.; Lucas Urraca, J.; Antonio Serrano, L.; Orellana, G.; Cruz Moreno-Bondi, M. Simultaneous determination of zearalenone and alternariol mycotoxins in oil samples using mixed molecularly imprinted polymer beads. Food Chem. 2023, 412, 135538 DOI:10.1016/j.foodchem.2023.135538.

Abstract

This work reports the optimization of a method using Molecularly Imprinted Polymers (MIPs) for the simultaneous determination of zearalenone and alternariol mycotoxins. The method was optimized using a chemometric approach where in the optimized conditions, the cartridges with a mixture (50:50, w/w) of both MIPs, were loaded with 30 mL of sample, washed with 2 mL of ACN/water (20/80, v/v) and eluted with 2.5 mL of trifluoroacetic acid/MeOH (3/97, v/v). The extracts were analyzed by HPLC coupled to a fluorescence detector (FLD). The optimized method has been applied and validated to the analysis of the mycotoxins in maize, sunflower and olive oils samples with a limit of detection of 5 and 2 μg kg- 1, respectively. Recoveries were in the range of 94 % to 108 % (RSD < 6 %) for zearalenone and 92 % to 113 % (RSD < 5 %) for alternariol. The results were confirmed by HPLC-MS/MS.