Strength and medium-term impact of HisAK70 immunization in dogs: Vaccine safety and biomarkers of effectiveness for ex vivo Leishmania infantum infection

Martínez Rodrigo, Abel; Mas Zubiri, Alicia; Fernández-Cotrina, Javier; Belinchón-Lorenzo, Silvia; Orden Gutiérrez, José Antonio; Arias, Pablo; Fuente López, Ricardo De La; Carrión Herrero, Francisco Javier; Domínguez Bernal, Gustavo Ramón

Strength and medium-term impact of HisAK70 immunization in dogs: Vaccine safety and biomarkers of effectiveness for ex vivo Leishmania infantum infection

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Official URL

https://doi.org/10.1016/j.cimid.2019.05.009

Publication date

2019

Authors

Martínez Rodrigo, Abel

Mas Zubiri, Alicia

Fernández-Cotrina, Javier

Belinchón-Lorenzo, Silvia

Orden Gutiérrez, José Antonio

Arias, Pablo

Fuente López, Ricardo De La

Carrión Herrero, Francisco Javier

Domínguez Bernal, Gustavo Ramón

Publisher

Elsevier

Citations

Exportar

URI

https://hdl.handle.net/20.500.14352/92568

Citation

Molina-Rueda F, Fernández-González P, Cuesta-Gómez A, Koutsou A, Carratalá-Tejada M, Miangolarra JC. Test-Retest Reliability of a Conventional Gait Model for Registering Joint Angles during Initial Contact and Toe-Off in Healthy Subjects. Int J Environ Res Public Health. 2021 Feb 15;3(18):1343-52

Abstract

HisAK70 candidates have successfully been tested in cutaneous (CL) and visceral leishmaniosis (VL) mouse models. Here, we analyse different biomarkers in dog trials after a heterologous immunization strategy with a HisAK70 candidate (plasmid DNA plus adoptive transfer of peripheral blood-derived dendritic cells (DCs) pulsed with the same pathoantigen and CpG ODN as an adjuvant) to explore the antileishmanial activity in an ex vivo canine co-culture system in the presence of Leishmania infantum parasites. In the canine model, the heterologous HisAK70 vaccine could decrease the infection index in the DC-T cell co-culture system by up to 54% after 30 days and reach almost 67% after 100 days post-immunization, respectively, compared to those obtained in the control group of dogs. The observed security and potential to ﬁght ex vivo L. infantum infection highlight a HisAK70 heterologous immunization strategy as a promising alternative to evaluate its effectiveness against canine VL.