Combination of bioanalytical approaches and quantitative proteomics for the elucidation of the toxicity mechanisms associated to TiO2 nanoparticles exposure in human keratinocytes
No Thumbnail Available
Full text at PDC
Publication date
2019
Advisors (or tutors)
Editors
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Citations
Exportar
Citation
Montalvo-Quiros, S.; Luque-Garcia, J. L. Combination of bioanalytical approaches and quantitative proteomics for the elucidation of the toxicity mechanisms associated to TiO2 nanoparticles exposure in human keratinocytes. Food and Chemical Toxicology 2019, 127, 197-205 DOI:10.1016/j.fct.2019.03.036.
Abstract
Titanium dioxide nanoparticles (TiO2-NPs) are being used in several consumer products. The high refractive index of nano-scaled titanium dioxide particles allows them to protect from UV radiation, and so, they can be found as one of the main components of cosmetics and suncreens. Many studies have reported the potential toxicological effects associated to TiO2-NPs such as ROS generation, DNA damage, apoptosis and cell cycle arrest, among others. The continuous and systematic use of TiO2-NPs in cosmetic products requires a full comprehension of the risks involving their sustained contact with the human skin. Thus, it is important to evaluate not only the hazardous effects but to elucidate the biomolecular mechanisms involved in such effects. Based on this premises, we have evaluated the potential toxicity of TiO2-NPs using a human epithelial cell culture (HaCaT cells) as in-vitro model, together with different bioanalytical approaches and mass spectrometry-based quantitative proteomics, to gain a deeper insight into the molecular mechanisms of toxicity associated to TiO2-NPs exposure.